TROPHIC MATERIAL IN NEURONAL TISSUE

神经组织中的营养物质

• 批准号: 3859975
• 负责人: J ZHU
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF MENTAL HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3859975
• 关键词: adrenal glands; adrenal medulla; axon; chromaffin cells; dendrites; growth factor; laboratory rat; molecular genetics; neurochemistry; protein purification; tissue /cell culture

During the development of a primary culture of chromaffin cells from adult rat adrenals, neurite outgrowth from chromaffin cells was sometimes observed. Subsequently we collected the media in which neurite outgrowth was observed in chromaffin cells and found that it stimulated neurite outgrowth activity. In this study, the culture conditions that lead to a generation of the neurite outgrowth promoting activity were established. We found that if dissociated adrenal medullary cells were first cultured in cytosine arabinoside-free media to allow non-chromaffin cells to proliferate and then in cytosine arabinoside containing media, the neurite outgrowth from chromaffin cells was observed. Furthermore, media collected one week after the addition of cytosine arabinoside were found to promote neurite outgrowth activity. The potency of the neurite outgrowth activity appeared to depend on the number of non-chromaffin cells in the culture, suggesting that non-chromaffin cells may be the source of the neurite outgrowth activity. This neurite outgrowth activity was found to be a protein, but is distinct from NGF, aFGF, bFGF, EGF, and IGF-I. We are currently preparing a large quantity of adrenal medullary cells for extraction of this neurite outgrowth promoting factors for further characterization.

在成人嗜铬细胞原代培养的发育过程中， 在大鼠肾上腺中，嗜铬细胞的神经突生长有时 观察 随后，我们收集了神经突生长的培养基， 在嗜铬细胞中观察到， 副产物活动。 在这项研究中，培养条件，导致一个 神经突生长促进活性的产生。 我们发现，如果分离的肾上腺髓质细胞首先培养在 不含阿糖胞苷的培养基， 然后在含有阿糖胞苷的培养基中， 观察到嗜铬细胞的生长。 此外，媒体收集 添加阿糖胞苷一周后发现， 神经突生长活性。 神经突生长活性的潜力 似乎取决于培养物中非嗜铬细胞的数量， 这表明非嗜铬细胞可能是神经突的来源 副产物活动。 这种神经突生长活动被发现是一种 蛋白，但不同于NGF、aFGF、bFGF、EGF和IGF-I。 我们 目前正在准备大量的肾上腺髓质细胞， 提取这种神经突生长促进因子， 特征化