TROPHIC MATERIAL IN NEURONAL TISSUE

成人嗜铬细胞原代培养的发育过程 大鼠肾上腺，嗜铬细胞突起有时可见 观察到的。随后，我们收集了轴突生长的培养液。 在嗜铬细胞中观察到，并发现它刺激了轴突 外生活动。在这项研究中，导致 建立了神经突起的生长促进活性。 我们发现，如果分离的肾上腺髓质细胞首先在 不含阿糖胞苷的培养液允许非嗜铬细胞 然后在含有阿糖胞苷的培养液中增殖，突起 观察到嗜铬细胞的突起。此外，收集的媒体 一周后加入胞嘧啶阿拉伯糖苷被发现促进 轴突生长活动。神经突起生长活动的效力 似乎取决于培养物中非嗜铬细胞的数量， 提示非嗜铬细胞可能是突起的来源。 外生活动。这种突起生长活动被发现是一种 蛋白质，但不同于NGF、aFGF、bFGF、EGF和IGF-I。我们是 目前正在大量制备肾上腺髓质细胞用于 提取这种神经突起生长促进因子，为进一步 人物刻画。

During the development of a primary culture of chromaffin cells from adult rat adrenals, neurite outgrowth from chromaffin cells was sometimes observed. Subsequently we collected the media in which neurite outgrowth was observed in chromaffin cells and found that it stimulated neurite outgrowth activity. In this study, the culture conditions that lead to a generation of the neurite outgrowth promoting activity were established. We found that if dissociated adrenal medullary cells were first cultured in cytosine arabinoside-free media to allow non-chromaffin cells to proliferate and then in cytosine arabinoside containing media, the neurite outgrowth from chromaffin cells was observed. Furthermore, media collected one week after the addition of cytosine arabinoside were found to promote neurite outgrowth activity. The potency of the neurite outgrowth activity appeared to depend on the number of non-chromaffin cells in the culture, suggesting that non-chromaffin cells may be the source of the neurite outgrowth activity. This neurite outgrowth activity was found to be a protein, but is distinct from NGF, aFGF, bFGF, EGF, and IGF-I. We are currently preparing a large quantity of adrenal medullary cells for extraction of this neurite outgrowth promoting factors for further characterization.