SECRETION OF NEUROPEPTIDE (FLFQPQRF-NH2) ENDOGENOUS ANTIOPIOID PEPTIDES

神经肽 (FLFQPQRF-NH2) 内源性抗阿片肽的分泌

• 批准号: 3845303
• 负责人: J ZHU
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF MENTAL HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3845303
• 关键词: analgesics; drug withdrawal; laboratory rat; morphine; naloxone; narcotic antagonists; neural transmission; neuropeptide receptor; neuropeptides; secretory protein; spinal cord mapping; substance P

NeuropeptideFF (FLFQPQRF-NH2) is an endogenous neuropeptide with morphine modulating activity. Neuropeptide FF (NPFF) immunoreactive nerve terminals and NPFF receptors were found to be highly localized in the superficial laminae of rat dorsal spinal cords. These observations strongly suggest a role for NPFF in the spinal cord function. In order to explore the functional role of NPFF, the secretion of NPFF from the rat spinal cord was studied. Furthermore, the possible effects of opiates on this NPFF release were also investigated. Substance P caused a release of NPFF from the spinal cord in a calcium dependent manner. Further studies with other substance P related peptides used as secretagogues indicated that a specific substance P receptor (SP-N) is involved in NPFF secretion from the spinal cord. In view of the role of substance P in sensory transmission, the results of this study strongly suggest a modulatory role for NPFF in antinociception. The study on the effect of morphine on NPFF efflux has demonstrated that naloxone can induce a release of NPFF from the spinal cord of rats pretreated with morphine. In contrast, naloxone was found to have nearly no effect on the basal efflux of NPFF. Other investigators have previously reported that NPFF administered ICV (third ventricle) into morphine-dependent rats can precipitate an opiate abstinence syndrome. Thus the results of this study strongly indicate that NPFF may be involved in naloxone precipitated abstinence syndrome in morphine dependent animals.

神经肽FF（NeuropeptideFF，FLPQRF-NH 2）是一种与吗啡结合的内源性神经肽 调节活动。 神经肽FF（NPFF）免疫反应性神经 末端和NPFF受体被发现高度定位于 大鼠背侧脊髓浅层。 这些观察结果 强烈提示NPFF在脊髓功能中的作用。 为了 为了探讨NPFF的功能作用， 对大鼠脊髓进行了研究。 此外， 还研究了阿片类药物对该NPFF释放的影响。 P物质引起的 以钙依赖性方式从脊髓释放NPFF。 使用其他P物质相关肽作为 促分泌素表明，一个特定的P物质受体（SP-N）， 参与脊髓分泌NPFF。 鉴于联合国的作用， P物质在感觉传递中的作用，这项研究的结果强烈 提示NPFF在抗伤害感受中调节作用。 的研究 吗啡对NPFF外排的影响已经证明纳洛酮可以 诱导NPFF从用NPFF预处理的大鼠的脊髓释放 吗啡 相比之下，纳洛酮几乎没有影响， NPFF的基础外排。 其他调查人员此前曾报道， NPFF给吗啡依赖大鼠第三脑室（ICV） 会引发阿片类药物戒断综合症 因此， 研究强烈表明NPFF可能参与纳洛酮 在吗啡依赖动物中诱发戒断综合征。