SECRETION OF FMRF-AMIDE LIKE PEPTIDES, ENDOGENOUS ANTIOPIOD PEPTIDES

FMRF-酰胺样肽、内源性抗碘肽的分泌

• 批准号: 3859976
• 负责人: J ZHU
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF MENTAL HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3859976
• 关键词: dynorphins; laboratory rat; molecular genetics; morphine; naloxone; neural transmission; neuropeptide receptor; neuropeptides; neurophysiology; norepinephrine; pain; secretion; serotonin; spinal cord mapping; substance K; substance P

The neuropeptide FLFQPQRF-NH2 (F-8-F-NH2) was initially isolated from the bovine brain and found to have morphine modulating activity. F-8-F-NH2 immunoreactive terminals and F-8-F-NH2 receptors were found to be highly localized in the superficial laminae of rat dorsal spinal cords. These observations strongly suggest a role for F-8-F-NH2 in the spinal cord function. In order to explore the functional role of F-8-F-NH2, in this study effects of various transmitters on the secretion of F-8-F-NH2 and the possible interactions between F-8-F-NH2 and opiate mediated responses were investigated in rat spinal cord. Substance P induced the secretion of F-8- F-NH2 from the spinal cord while substance K, norepinephrine and serotonin failed to show such an effect. To explore the possible interaction between F-8-F-NH2 and opiate mediated responses, the secretions of dynorphin and F- 8-F-NH2 from rat spinal cords were studied. The 56 mM KCl evoked secretion of dynorphin was significantly inhibited by the addition of F-8-F-NH2 into the perfusion medium. The 56 mM KCl induced secretion of F-8-F-NH2 was significantly reduced by the addition of naloxone and morphine together into the perfusion medium potentiated the 56 mM KCl evoked F-8-F-NH2 secretion while morphine or naloxone alone showed little effect on the F-8- F-NH2 secretion. The results of this study suggest that F-8-F-NH2 may have a role in the nociceptive transmission and, furthermore, there may be a modulatory role for F-8-F-NH2 in opiate mediated responses.

神经肽FLIPQRF-NH 2（F-8-F-NH 2）最初是从神经细胞中分离的。 牛脑，并发现具有吗啡调节活性。 F-8-F-NH2 免疫反应终末和F-8-F-NH 2受体被发现是高度 定位于大鼠背侧脊髓的浅层。 这些 观察结果强烈表明F-8-F-NH 2在脊髓中的作用 功能 为了探讨F-8-F-NH 2的功能作用， 研究不同递质对F-8-F-NH_2分泌的影响， F-8-F-NH 2和阿片介导的反应之间可能的相互作用是 在大鼠脊髓中研究。 P物质诱导F-8- 而K物质、去甲肾上腺素和5-羟色胺 没有表现出这样的效果。 为了探索 F-8-F-NH 2和阿片介导的反应，强啡肽和F-8的分泌 对大鼠脊髓中的8-F-NH_2进行了研究。 56 mM KCl诱发分泌 F-8-F-NH_2对强啡肽的合成有明显的抑制作用。 灌注介质。 56 mM KCl诱导的F-8-F-NH 2分泌为 纳洛酮和吗啡同时加入可显著降低 56 mM KCl诱发的F-8-F-NH 2 而吗啡或纳洛酮单独对F-8- F-NH 2分泌。 这项研究的结果表明，F-8-F-NH 2可能具有 在伤害性传递中的作用，此外，可能存在 F-8-F-NH 2在阿片介导的反应中的调节作用。