SECRETION OF FMRF-AMIDE LIKE PEPTIDES--ENDOGENOUS ANTIOPIOD PEPTIDES

神经肽Phe-Leu-Phe-Gln-Pro-Gln-Arg-Phe-NH，(F-8-F-NH2)是 从牛脑中分离出具有吗啡调节活性的化合物 当大鼠集中给药时。在脊髓中，这种多肽是 高度集中在背角，储存在神经中 胶状质中的终末。在这项研究中，分泌物 对F-8-F-NH2进行了研究。采用体外灌流技术 分离完整的脊髓，发现F-8-F-NH2可以释放 通过以钙依赖的方式去极化Kc1的浓度。这 吗啡可减弱F-8-F-NH2的诱导释放。 灌流介质。吗啡的作用呈剂量依赖关系。 这项研究的结果与以下假设很好地一致 F-8-F-NH2可能在阿片类药物介导的抗伤害效应中起调制作用。 这项研究的建议过程是调查生理上的 正常大鼠和吗啡耐受依赖大鼠F-8-F-NH2的释放 发送器对F-8-F-NH2的生理分泌也将是 调查过了。这项研究的目的是探索可能的作用 F-8-F-NH2在阿片类药物依赖或耐受的形成中的作用。

The neuropeptide, Phe-Leu-Phe-Gln-Pro-Gln-Arg-Phe-NH, (F-8-F-NH 2) was isolated from bovine brain and found to have morphine modulating activity when administered centrally to the rat. In the spinal cord, this peptide is highly concentrated in the dorsal horn where it is stored in nerve terminals in the substantia gelatinosa. In this study, the secretion of F-8-F-NH 2 was investigated.By an in vitro superfusion technique using isolated intact spinal cords,it was found that F-8-F-NH 2 could be released by depolarizing concentrations of KC1 in a calcium dependent manner. This induced release of F-8-F-NH 2 was attenuated by addition of morphine to the superfusion medium. The effect of morphine was found to be dose dependent. The result of this study is in good agreement with the hypothesis that F-8-F-NH 2 may have a modulatory role in opioid mediated antinociception. The proposed course of this study is to investigate the physiological release of F-8-F-NH 2 in normal and morphine tolerant or dependent rats. The physiological secretion of F-8-F-NH 2 by transmitters will also be investigated. The aim of this study is to explore the possible role of F-8-F-NH 2 in the development of opiate dependence or tolerance.