SECRETION OF FMRF-AMIDE LIKE PEPTIDES--ENDOGENOUS ANTIOPIOD PEPTIDES

FMRF-酰胺样肽的分泌--内源性抗碘肽

• 批准号: 3880994
• 负责人: J ZHU
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF MENTAL HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3880994
• 关键词: cow; dosage; drug tolerance; laboratory rat; molecular genetics; morphine; opiate alkaloid; secretion

The neuropeptide, Phe-Leu-Phe-Gln-Pro-Gln-Arg-Phe-NH, (F-8-F-NH 2) was isolated from bovine brain and found to have morphine modulating activity when administered centrally to the rat. In the spinal cord, this peptide is highly concentrated in the dorsal horn where it is stored in nerve terminals in the substantia gelatinosa. In this study, the secretion of F-8-F-NH 2 was investigated.By an in vitro superfusion technique using isolated intact spinal cords,it was found that F-8-F-NH 2 could be released by depolarizing concentrations of KC1 in a calcium dependent manner. This induced release of F-8-F-NH 2 was attenuated by addition of morphine to the superfusion medium. The effect of morphine was found to be dose dependent. The result of this study is in good agreement with the hypothesis that F-8-F-NH 2 may have a modulatory role in opioid mediated antinociception. The proposed course of this study is to investigate the physiological release of F-8-F-NH 2 in normal and morphine tolerant or dependent rats. The physiological secretion of F-8-F-NH 2 by transmitters will also be investigated. The aim of this study is to explore the possible role of F-8-F-NH 2 in the development of opiate dependence or tolerance.

神经肽Phe-Leu-Phe-Gln-Pro-Gln-Arg-Phe-NH，（F-8-F-NH 2）为 从牛脑中分离并发现具有吗啡调节活性 当向大鼠中枢给药时。在脊髓中，这种肽 高度集中在背角，储存在神经中 终末在胶状质中。在这项研究中， 采用体外灌流技术， 在离体完整脊髓中，发现F-8-F-NH 2可释放， 通过以钙依赖性方式去极化KCl浓度。这 吗啡可使F-8-F-NH 2的诱导释放减弱。 灌流介质吗啡的作用呈剂量依赖性。 本研究的结果与以下假设相吻合： F-8-F-NH 2可能在阿片介导的抗伤害作用中起调节作用。 这项研究的目的是研究生理 正常大鼠和吗啡耐受或依赖大鼠F-8-F-NH 2的释放。 通过递质的生理分泌F-8-F-NH 2也将是 研究了本研究的目的是探讨 F-8-F-NH 2与阿片类药物依赖或耐受的发生