ROTATING ANODE X-RAY DIFFRACTION SYSTEM

旋转阳极 X 射线衍射系统

• 批准号: 3521305
• 负责人: W. ROBERT SCHEIDT
• 金额: $ 20.2万
• 依托单位: UNIVERSITY OF NOTRE DAME
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-06-02 至 1992-06-01
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3521305
• 关键词: X ray crystallography; antibiotics; biological products; biomedical equipment purchase; biomedical equipment resource; chemical structure; chemistry; conformation; metalloporphyrins; nucleosides; online computer; siderophores

Funds for the purchase of a modern X-ray diffraction system (consisting of a rotating anode X-ray source, diffractometer, associated computer and graphics system) are requested. This X-ray diffraction system will be used to collect data, solve, refine and graphically depict structural results in support of several NIH-supported projects. The requested hardware will enhance structure determination capabilities by providing a more intense X-ray source to reduce data collection times, improve signal to noise ratios for marginal crystalline specimens and allow for more careful preliminary examination of crystals. Molecular structure determinations are planned for a variety of chemical species related to natural products and biochemical systems. Studies of metalloporphyrin complexes will relate basic structural and physicochemical data to the function of hemoproteins that are fundamental to a wide range of biological processes including oxygen utilization and transport, electron transport, drug metabolism and other enzymatic processes. The molecular structure and solid-state conformations of modified nucleosides will be studied in order to assess the effect of furanose ring structure on overall conformation. Structure determinations will be performed to support new synthetic methods in the synthesis of streptogramin and Beta-lactam antibiotics. Such studies have proved to be important in understanding biological activity, provide structural evidence of synthetic intermediates, and suggest alternate synthetic routes to yield desired products. Structures of siderophores and siderophore-antibiotic conjugates are expected to provide a better understanding of the active and inactive forms of these drug and drug-delivery systems. Molecular structure determination will also be performed in support of research programs concerned with the development of new synthetic methods of pharmacological significance.

购买现代X射线衍射系统的资金(包括 旋转阳极X射线源、衍射仪、相关计算机 和图形系统)。这套X射线衍射系统将 用于收集数据、求解、细化和以图形方式描述结构 结果支持了美国国立卫生研究院支持的几个项目。所请求的 硬件将通过提供以下功能增强结构确定能力 更强的X射线源可减少数据收集时间，提高 边缘晶体样品的信噪比，并允许 对晶体进行更仔细的初步检查。分子结构 计划对与以下物质相关的各种化学物种进行检测 天然产物和生化系统。金属卟啉的研究进展 络合物将把基本的结构和物理化学数据与 血色素蛋白的功能，它是广泛的 包括氧气利用和运输在内的生物过程， 电子传递、药物代谢等酶过程。这个 改性产物的分子结构和固态构象 将对核苷进行研究，以评估呋喃糖的作用。 整体构象上的环状结构。结构确定将 在合成中支持新的合成方法 链菌素和β-内酰胺类抗生素。这些研究已经证明 在理解生物活性方面很重要，提供结构 合成中间体的证据，并建议替代合成中间体 生产所需产品的路线。铁载体的结构和 铁载体-抗生素结合物有望提供更好的 对这些药物的活性和非活性形式的理解 药物输送系统。分子结构的测定也将是 执行以支持与开发相关的研究计划 具有药理意义的新合成方法。