AUTORADIOGRAPH IMAGER

由六名酶学家和分子生物学家组成的主要用户组 马里兰大学化学和生物化学系 学院公园为一台自动放射成像仪寻求资金；主要用户 小组由阿姆斯特朗博士、达纳韦-马里亚诺博士、格尔特(P.I.)、 汉森、朱林和科萨里奇。这台成像仪将被用来代替 DNA损伤定量的传统放射自显影方法 多种抗生素和酶，定量Southern和Northern印迹 突变基因的分析和DNA序列分析，其中一些是 显著富含GC。成像器减少了获取 描述凝胶和过滤器上放射性分布的数据应 与X射线相比，它的荧光屏的灵敏度提高了 电影。与成像器一起提供的软件还可以进行定量 对凝胶和过滤器不同区域的数据进行处理和比较。 激光打印机提供的凝胶或滤光片的硬拷贝为 适用于出版，也将有助于准确阅读 DNA测序凝胶。

A major users group of six enzymologists and molecular biologists in the Department of Chemistry and Biochemistry of the University of Maryland at College Park seeks funding for an autoradiograph imager; the major users group is composed of Drs. Armstrong, Dunaway-Mariano, Gerlt (P.I.), Hansen, Julin, and Kozarich. This imager will be used in lieu of traditional autoradiographic methods for quantitation of DNA damage by various antibiotics and enzymes, quantitative Southern and Northern blot analyses, and DNA sequence analyses of mutant genes, some of which are significantly GC rich. The imager decreases the time required to obtain data describing the distribution of radioactivity on gels and filters due to the enhanced sensitivity of its phosphor screens as compared to X-ray film. The software provided with the imager also allows quantitative treatment and comparisons of data on different regions of gels and filters. The hard copy of the gel or filter which is provided by a laser printer is suitable for publication and also will facilitate the accurate reading of DNA sequencing gels.