GENOMIC ENZYMOLOGY: THE ENOLASE SUPERFAMILY AND OMPDC SUPRAFAMILY

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 我们的研究集中在理解的结构基础上，不同的进化组的同源酶，催化不同的反应。 我们感兴趣的烯醇化酶超家族，一组机械多样的酶，共享烯醇化的羧酸阴离子底物，乳清酸核苷5-单磷酸（OMPDC）超家族，一组功能不同的酶，共享没有可辨别的机械属性，和D-核酮糖1，5-二磷酸羧化酶/加氧酶超家族。 所有三个同源酶组共享（B/a）8倍，这是结构特征酶中最常见的折叠。 目前，我们正在使用嵌合体来可视化和分析所有三个同源组的成员的结构。 我们还使用SFLD来协助我们的生物信息学分析。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our studies focus on understanding the structural bases for divergent evolution in groups of homologous enzymes that catalyze different reactions. We are interested in the enolase superfamily, a group of mechanistically diverse enzymes that share enolization of a carboxylate anion substrate, and the orotidine 5-monophosphate (OMPDC) suprafamily, a group of functionally distinct enzymes that share no discernible mechanistic attributes, and D-ribulose 1,5-bisphosphate carboxylase/oxygenase superfamily. All three homologous groups of enzymes share the (b/a)8-fold, the most commonly observed fold in structurally characterized enzymes. At present we are using Chimera to visualize and superimpose structures of members of all three homologous groups. We also use the SFLD to assist with our bioinformatic analyses.