GENOMIC ENZYMOLOGY: THE ENOLASE SUPERFAMILY AND OMPDC SUPRAFAMILY

基因组酶学：烯醇化酶超家族和 OMPDC 超家族

• 批准号: 8170502
• 负责人: JOHN A GERLT
• 金额: $ 1.79万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170502
• 关键词: Anions; Bioinformatics; Chimera organism; Computer Retrieval of Information on Scientific Projects Database; Enzymatic Biochemistry; Enzymes; Evolution; Funding; Genomics; Grant; Institution; Oxygenases; Reaction; Research; Research Personnel; Resources; Source; Structure; United States National Institutes of Health; base; carboxylate; enolase; interest; member; orotidylic acid; ribulose

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our studies focus on understanding the structural bases for divergent evolution in groups of homologous enzymes that catalyze different reactions. We are interested in the enolase superfamily, a group of mechanistically diverse enzymes that share enolization of a carboxylate anion substrate, and the orotidine 5-monophosphate (OMPDC) suprafamily, a group of functionally distinct enzymes that share no discernible mechanistic attributes, and D-ribulose 1,5-bisphosphate carboxylase/oxygenase superfamily. All three homologous groups of enzymes share the (b/a)8-fold, the most commonly observed fold in structurally characterized enzymes. At present we are using Chimera to visualize and superimpose structures of members of all three homologous groups. We also use the SFLD to assist with our bioinformatic analyses.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 我们的研究重点是了解催化不同反应的同源酶组中趋异进化的结构基础。 我们对烯醇化酶超家族感兴趣，这是一组机制多样的酶，它们共享羧酸根阴离子底物的烯醇化，以及乳清苷5-单磷酸（OMPDC）超家族，一组功能不同的酶，没有明显的机制属性，以及D-核酮糖1,5-二磷酸羧化酶/加氧酶超家族。 所有三个同源酶组都具有 (b/a)8 折叠，这是结构特征酶中最常见的折叠。 目前，我们正在使用 Chimera 来可视化和叠加所有三个同源组成员的结构。 我们还使用 SFLD 来协助我们的生物信息学分析。