Bridging Project 3: Glutathione Transferase (GST) Superfamily

桥接项目 3：谷胱甘肽转移酶 (GST) 超家族

• 批准号: 7980209
• 负责人: JOHN A GERLT
• 金额: $ 30.12万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-20 至 2015-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7980209
• 关键词: Academic Medical Centers; Aerobic; Bacteria; Binding; Biological Process; Biology; C-terminal; Classification; Computer Simulation; Consensus; Crystallization; Disulfides; Docking; Drug Metabolic Detoxication; Epoxy Compounds; Escherichia coli; Eukaryota; Evaluation; Family member; Genetic Screening; Genome; Glutathione S-Transferase; Homeostasis; Homologous Gene; Hydrogen Peroxide; Hydrolysis; Ions; Knowledge; Libraries; Ligands; Mammals; Membrane; Microbiology; Mission; N-terminal; Oranges; Organism; Oxidation-Reduction; Play; Production; Proteins; Reaction; Role; Scanning; Structure; Sulfhydryl Compounds; Thioredoxin; To specify; Transcriptional Regulation; Transferase; Translations; Xenobiotics; aryl halide; detoxication; dimer; functional group; genome sequencing; high throughput screening; knockout gene; member; microorganism; protein folding; protein function; protein protein interaction; protein structure; selenate; virtual

The GST superfamily is a large group of proteins, the members of which are composed of two domains, an N-terminal thioredoxin-like domain (orange) that binds GSH, and a C-terminal all a-helical domain (blue), as illustrated by the structure of the GST protein from ¿. coli in the figure to the right [1, 2). These proteins are found in most aerobic organisms. The GSTs were discovered in mammals over forty-five years ago [3]. For the first thirty years, their role in biology was thought to lie exclusively in the detoxification of endogenous and xenobiotic compounds by catalyzing the addition of GSH to electrophilic functional groups, as illustrated in Eq. 1. These reactions include electrophiles such as epoxides, enones and alkyl and aryl halides The catalytic diversity of GSTs also includes isomerization reactions, hydrolysis, and redox reactions with organic hydroperoxides, disulfides and, perhaps, selenides and selenates. The functional diversity of these proteins exceeds their catalytic promiscuity. In bacteria and eukaryotes, the proteins are involved in detoxication reactions, catabollsm, the regulation of transcription and translation, the conductance of ions across membranes, and (we are almost sure) thiol homeostasis and protein folding. Nine of the approximately 4,400 proteins encoded by the ¿. coli genome encode GST homologs [2, 4). The consensus residues for the GST homologs found in E. coli are shown in space-filling representation in the structure above to highlight the fact that the highly conserved residues in GST family members are principally involved in the structural stability of the fold rather than the function of the protein. The principal role of the thioredoxin-like domain is to bind GSH or related ligands while the a-helical domain tends to specify more subtle aspects ofthe protein function. The proteins are typically dimers.

GST超家族是一大群蛋白质，其成员由两个结构域组成，一个结合GSH的n端硫氧还蛋白样结构域（橙色）和一个c端全a-螺旋结构域（蓝色），如图中GST蛋白的结构所示。右边图中的大肠杆菌[1,2]。这些蛋白质存在于大多数有氧血液中