COMPARATIVE ASPECTS OF IONIC CONDUCTANCES IN NERVE AND HEART CELL MEMBRANES

神经细胞和心脏细胞膜中离子电导的比较

• 批准号: 3881743
• 负责人: J R CLAY
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3881743
• 关键词: Cephalopoda; action potentials; adenosine triphosphate; arrhythmia; atrium; axon; biological models; calcium channel; cell membrane; chick embryo; computer simulation; electrophysiology; embryo /fetus cell /tissue; extracellular; guinea pigs; heart cell; heart ventricle; ion transport; membrane channels; membrane permeability; membrane potentials; membrane transport proteins; microelectrodes; model design /development; muscle cells; neural conduction; neurophysiology; phosphorylation; saltwater environment; single cell analysis; transport proteins

This project is concerned with a comparative analysis of ionic current channels in nerve and heart cell membranes and the relationship of these channels to electrical excitability in both preparations. During the past year the primary experimental preparations have been squid giant axons and embryonic chick heart cells. One major finding has been the discovery of Wenkebach firing patterns in the squid axon in response to periodic current pulse stimulation. This phenomenon, which has not previously been characterized in this preparation, may be relevant to cardiac arrhythmias. In the chick heart cell preparation, we have discovered a developmental loss of a particular type of calcium ion channel, the IT component, during the first week of embryonic development from atrial and ventricular heart cells. This project also has a major emphasis on computer simulations of electrical activity in nerve and heart cells. A major advance in this part of the project is the development of a model of the action potential in guinea-pig ventricular myocytes based on experimental measurements of underlying ion current components in the literature.

本课题是关于离子电流的比较分析 神经和心脏细胞膜中的通道以及它们之间的关系 通道的电兴奋性在这两个准备。在过去 一年来，主要的实验准备工作一直是鱿鱼巨大的轴突， 鸡胚心脏细胞一个主要的发现是 鱿鱼轴突对周期性应激反应的Wenkebach放电模式 电流脉冲刺激这种现象以前从未出现过， 其特征在于，可能与心律失常有关。 在鸡心脏细胞制备中，我们发现了一种发育 一种特殊类型的钙离子通道，IT组件的损失， 胚胎发育的第一周，从心房和心室心脏 细胞这个项目也有一个主要的重点是计算机模拟， 神经和心脏细胞的电活动。这一部分的一个重大进步是 该项目的主要目的是开发一个动作电位模型， 豚鼠心室肌细胞的基础上的实验测量， 在文献中的基本离子电流分量。