ANALYSIS OF ION CHANNELS IN AXOPLASMIC ORGANELLES

轴浆细胞器离子通道分析

• 批准号: 3782341
• 负责人: J R CLAY
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3782341
• 关键词: alternatives to animals in research; axoplasm; chromatography; gel electrophoresis; ion transport; lipid bilayer membrane; membrane channels; neurofilament; neuronal transport; organelles; potassium channel; sodium channel; transmission electron microscopy; voltage /patch clamp

This project is concerned with axoplasmic organelles containing sodium and potassium ion channels which are transported along the axon via neurofilaments to various points where they fuse with the axonal membrane, thereby inserting ion channels which underlie excitability in the membrane. We have recently found that the organelles can be separated on the basis of size into anterograde and retrograde fractions via control- pore-size glass bead chromatography. We are currently investigating these two fractions with transmission electron microscopy and SDS polyacrylamide gel electrophoresis. We are also investigating the ion channels contained in each fraction by incorporating the organelles into artificial lipid bilayers and using the voltage-clamp technique to characterize the ion channels electrophysiologically.

本项目涉及含钠的轴浆细胞器， 钾离子通道，其通过以下途径沿着轴突运输 将神经丝连接到它们与轴突膜融合的各个点， 从而将作为兴奋性基础的离子通道插入到细胞中， 膜的 我们最近发现，细胞器可以分离， 通过控制将大小分为顺行和逆行分数- 孔径玻璃珠色谱法。 我们目前正在调查这些 透射电镜和SDS分析两个组分 聚丙烯酰胺凝胶电泳 我们也在调查 通过将细胞器整合到每个部分中所包含的通道中， 人工脂质双层，并使用电压钳技术， 表征离子通道的电生理学特性。