ORGANELLE TRANSPORT OF ION CHANNELS IN EXCITABLE CELLS

这项研究涉及神经轴浆中离子通道的运输。 胞内细胞器。这个项目之前的工作已经证明 从鱿鱼的轴浆中分离出不同种群的细胞器 巨大的轴突；一个假定的具有直径的顺行细胞器群 在40-60 nm范围内，并且假定的逆行种群具有 直径在100-150 nm范围内。在过去的一年里，各种 显微技术已经被应用于试图将 细胞器在各种不同的环境中。例如，激光共聚焦 显微镜最近被用来观察布朗运动。 细胞器被标记有荧光标记，德克萨斯红。 这些结果被用来确定相对产量 细胞器中的各种级分来自柱子，并还提供 关于细胞器大小的一些信息来自布朗运动。得克萨斯州 红色处理的制剂也被用来尝试可视化 外源细胞器沿微管的运动，可能还包括 肌动蛋白细丝在体外运动性测定中的应用 干涉对比显微镜技术，他们也被 向鱿鱼轴突注射压力以确定靶向 用荧光显微镜观察细胞器对轴突细胞膜的影响。

This project concerns the transport of ion channels in nerve axoplasm by intracellular organelles. Previous work in this project has demonstrated distinct populations of organelles isolated from the axoplasm of squid giant axons; a putative anterograde organelle population having diameters in the 40-60 nm range, and a putative retrograde population having diameters in the 100-150 nm range. During the past year various microscopic techniques have been applied in an attempt to visualize the organelles in various different settings. For example, laser confocal microscopy has recently been used to observe Brownian motion of organelles which had been labeled with the fluorescent marker, Texas Red. These results are being used to determine the relative yield of organelles in the various fractions from the column, and also to provide some information on organelle size from the Brownian motion. The Texas Red treated preparations are also being used in an attempt to visualize motility of exogenous organelles along microtubules and perhaps also actin filaments in an in vitro motility assay with the differential interference contrast microscopy technique, and they are also being pressure injected into squid axons to determine the targeting of organelles to the axonal membrane with a fluorescence microscope.