GENE REGULATION WITHIN THE NERVOUS SYSTEM

我们探讨了细胞特异性和发育调节基因的表达 神经系统内的蛋白质使用神经元特异性(NSE)和非 以神经元(NNE)烯醇化酶同工酶为模型。人脑基因与基因组 DNA文库的构建使得这些和其他大脑的基因 可以分离和鉴定特异的蛋白质。两者都使用 人类NSE和NNE的抗体和寡核苷酸探针、cDNA 已经被分离和测序。利用这些cdna的独特区域 克隆作为探针，发育和细胞特异性调节 这些蛋白质中的每一种的mRNA的出现都可以用in-in来研究。 原位杂交。人类每一种同工酶的染色体基因座 将会被确认。此外，人类基因组克隆的分离用于 这些蛋白质中的每一种都应该提供关于调节 神经元和神经胶质细胞特异性蛋白在细胞中的表达 人类神经系统在正常和疾病状态下的分化。 NSE对神经源性细胞系的正常特异性和 NSE的特定DNA探针的可用性应该会提供一个有用的 神经源性正常细胞和肿瘤细胞特性的探讨 血统。

We approached the cell-specific and developmentally regulated expression of proteins within the nervous system using the neuron specific (NSE) and non- neuronal (NNE) enolase isozymes as a model. Human brain cDNA and genomic DNA libraries were constructed so that the genes for these and other brain specific proteins could be isolated and characterized. Using both antibodies and oligonucleotide probes, cDNAs for both human NSE and NNE have been isolated and sequenced. Employing unique regions of these cDNA clones as probes, the developmentally and cell-specific regulated appearance of mRNA for each of these proteins can be investigated using in- situ hybridization. The human chromosome loci for each of these isozymes will be identified. In addition, the isolation of human genomic clones for each of these proteins should provide information on the regulation of expression of neuron and glial specific proteins during cell differentiation of the human nervous system in normal and disease states. The normal specificity of NSE for neural derived cell lines and the availability of specific DNA probes for NSE should provide a useful approach to the characterization of neural derived normal and tumor cell lineages.