PROMOTION OF MYOCARDIAL ANGIOGENESIS VIA DIRECT APPLICATION OF FGF TO HEART

通过直接将 FGF 应用于心脏促进心肌血管生成

• 批准号: 3879072
• 负责人: ELLIS UNGER
• 金额: --
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3879072
• 关键词: acids; angiography; arterioles; artery stenosis; collagen; dogs; fibroblast growth factor; hyperplasia; myocardial infarction; myocardial ischemia /hypoxia; vascular smooth muscle

We sought to determine the effect of acidic fibroblast growth factor (aFGF) on ischemic and normal myocardium, and to determine whether direct application of aFGF to the heart could promote angiogenesis. Dogs underwent placement of an ameroid constrictor on the left anterior descending coronary artery (LAD). Three weeks later, a left internal mammary artery pedicle (IMA) was positioned over the LAD territory. A gortex or collagen I sponge saturated with aFGF (N=12) or saline (N=4) was interposed between the pedicle and the heart. Weekly angiography of the IMA was performed in all dogs, but significant IMA to coronary collaterals were not demonstrable in any dog. Eight dogs had histologic evidence of non-transmural infarction in the LAD territory (5 aFGF, 3 control). Striking smooth muscle cell (SMC) hyperplasia was present in arterioles and small arteries exclusively in areas of subendocardial infarction in all of the aFGF-treated dogs but in none of the control dogs (p<0.05). Non-infarcted myocardium appeared normal in all dogs. Two additional dogs received a similar aFGF-sponge but no ameroid; no pathology was seen. Thus, aFGF appears to stimulate SMC hyperplasia in areas subjected to infarction. Both injury and exposure to aFGF may be required to cause vascular SMC proliferation in the heart.

我们试图确定酸性成纤维细胞生长因子（aFGF） 对缺血和正常心肌的影响，并确定是否直接 将aFGF应用于心脏可促进血管生成。犬进行 在左前降支上放置Ameroid缩窄器 冠状动脉（LAD）。三周后，左乳内动脉 椎弓根（IMA）定位在LAD区域上。一个gortex或胶原蛋白I 将用aFGF（N=12）或盐水（N=4）饱和的海绵插在 蒂和心脏每周进行IMA血管造影， 所有犬，但冠状动脉侧支的显著IMA未得到证实 在任何狗。8只狗有非透壁性梗死的组织学证据 在LAD地区（5例aFGF，3例对照）。平滑肌细胞（SMC） 小动脉和小动脉增生仅见于 在所有aFGF治疗的狗中， 对照组无（p<0.05）。非梗死心肌表现正常 在所有狗中。另外两只狗接受了类似的aFGF海绵，但没有 无色素;未观察到病理学。因此，aFGF似乎刺激SMC 梗塞区域的增生。伤害和暴露于 可能需要aFGF来引起心脏中的血管SMC增殖。