FMRF-AMIDE LIKE PEPTIDES IN CNS--FUNCTION AND DISTRIBUTION

中枢神经系统中的 FMRF-酰胺样肽--功能和分布

• 批准号: 3880881
• 负责人: H-Y T YANG
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF MENTAL HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3880881
• 关键词: Mollusca; analgesia; arginine vasopressin; brain; central nervous system neoplasms; chemical structure function; cow; immunochemistry; inhibitor /antagonist; laboratory rat; molecular genetics; morphine; naloxone; nerve endings; neural transmission; neuroendocrine system; neuropeptides; neuropharmacology; oligopeptides; opiate alkaloid; pain threshold; pituitary gland; protein purification; protein structure function; secretion; spinal cord

Neuropeptide, Phe-Leu-Phe-Gln-Pro-Gln-Arg-Phe-NH2 (F-8-F-NH2), was initially detected by the antisera raised against the molluscan cardioexcitatory peptide FMRF-NH2 and subsequently isolated from bovine brain. Accumulating evidence suggests that F-8-F-NH2 may have a role in modulating opiate effects. In this study, the functional role(s) of FMRF-NH2 immunoreactivity (IR) including F-8-F-NH2 was further investigated. I.C.V. infusion of IgG from F-8-F-NH2 antiserum (as a specific F-8-F-NH2, antagonist) significantly reduced the number of abstinence signs precipitated by naloxone in morphine-dependent rats. This result and the previous findings of other investigators, that F-8-F-NH, can precipitate withdrawal in morphine dependent rats, suggest that F-8-F-NH2 may be involved in abstinence syndrome. Studies on pituitary F-8-F-NH2-IR indicate that there may be an interaction between F-8-F-NH2 and Arg(8)- vasopressin. In Brattleboro and salt loaded rats, animals known to have modified AVP biosynthesis, secretions of F-8-F-NH2-IR from pituitaries were found to be increased. Furthermore, in the pituitary, the postnatal development of F-8-F-NH2 was found to be very similar to that of AVP. Mammalian FMRF-NH2-IR is composed of multiple molecular forms which are not identical to FMRF-NH2. In this study 3 additional FMRF-NH2-like peptides were purified from rat -spinal cords and determination of their 'structures is in progress. The proposed courses of this project are 1) to continue the study on the functional role of F-B-F-NH, in the pituitary and 2) to determine the structures and biological activities of the newly isolated FMRF-NH2-like peptides.

神经肽Phe-Leu-Phe-Gln-Pro-Gln-Arg-Phe-NH_2（F-8-F-NH_2）， 最初由针对软体动物产生的抗血清检测到 心脏兴奋肽FMRF-NH 2，随后从牛中分离 个脑袋越来越多的证据表明，F-8-F-NH 2可能在 调节鸦片的作用在这项研究中， 包括F-8-F-NH 2在内的FMRF-NH 2免疫反应性（IR）进一步增强。 研究了I. C. V.输注来自F-8-F-NH 2抗血清的IgG（作为免疫抑制剂）。 特异性F-8-F-NH 2，拮抗剂）显著减少了 纳洛酮诱发吗啡依赖大鼠戒断症状。这 结果和其他研究人员以前的发现，F-8-F-NH，可以 提示F-8-F-NH 2 可能与戒断综合征有关垂体F-8-F-NH_2-IR的研究 表明F-8-F-NH 2与Arg（8）-之间可能存在相互作用。 加压素在布拉特尔伯勒和盐负荷大鼠，动物已知有 垂体分泌F-8-F-NH 2-IR， 发现增加了。此外，在脑垂体中， 发现F-8-F-NH_2的发育与AVP的发育非常相似。 哺乳动物FMRF-NH 2-IR由多种分子形式组成， 与FMRF-NH 2相同。 在这项研究中，3个额外的FMRF-NH 2样肽 从大鼠脊髓中分离纯化并测定其结构 正在进行 本项目的建议课程是：1）继续研究 F-B-F-NH在垂体中的功能作用; 2）确定 新分离的FMRF-NH 2-like的结构和生物活性 缩氨酸