PHE-MET-ARE-PHE-NH2 LIKE PEPTIDES IN THE BRAIN AND SPINAL CORD

大脑和脊髓中的 PHE-MET-ARE-PHE-NH2 样肽

• 批准号: 3968492
• 负责人: H-Y T YANG
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF MENTAL HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3968492
• 关键词: affinity chromatography; analgesia; antiserum; bioassay; brain mapping; brain metabolism; cerebral cortex; chemical structure function; drug interactions; drug metabolism; hypothalamus; immunochemistry; neural transmission; neurochemistry; neuropharmacology; neurotransmitter metabolism; oligopeptides; pain threshold; peptide chemical synthesis; pituitary gland; radioimmunoassay; spinal cord

We have previously isolated and chemically characterized two putative endogenous opiate antagonist peptides. In this study, antibodies against these two peptides were produced and distribution of these two peptides in brain and spinal cord was investigated by radioimmunoassay and also immunohistochemical technique. The immunohistochemical study was carried out with the cooperation of Dr. Pertti Panula at the University of Helsinki, Finland. These two peptides were found to be unevenly distributed in the brain with the highest concentrations in dorsal spinal cord and periaqueductal gray area and the lowest levels in cortex and cerebellum. In the spinal cord, immunoreactivities were found in laminae I-II of the posterior horn. Immunohistochemically, several groups of neurons in the rat brain were immunoreactive; immunoreactive cells were found in the cerebral cortex, lateral septal nucleus of the diagonal band, neostriatum, periventricular hypothalamic areas and several medullary nuclei. The identification of the two putative endogenous opiate antagonist peptides in brain neurons suggest that these two peptides may have functions in neurotransmission. The enrichment of these two peptides in dorsal spinal cord and periaqueductal gray area together with our past findings led us to suggest that these two peptides may participate in pain modulation. The proposed courses of the study on the two putative endogenous opiate antagonist peptides are: (1) to investigate the mechanism underlying the antiopiate action (2) to determine the distribution of receptors and (3) to explore the possible role of these peptides in development of opioid tolerance.

我们之前已经分离并化学表征了两种假定的