PHE-MET-ARG-PHE-NH2 LIKE PEPTIDES IN THE BRAIN AND SPINAL CORD

八肽。 Phe-Leu-Phe-Gln-Arg-Phe-NH_2（F-8-F-NH_2）首先被合成， 通过抗软体动物的抗血清检测到 心脏兴奋肽 Phe-Met-Arg-Phe-NH 2，以及随后 从牛脑中分离出来的 F-8-F-NH 2脑室注射 可降低吗啡的镇痛作用。 发现F-8-F-NH 2 高度定位于脊髓背侧胶状质 绳子 在使用体外和体内灌注的研究中， 在脊髓中，我们发现F-8-F-NH 2可以通过 KCl的去极化浓度（体外）以及物质 P，感觉递质（体内）。 这一结果进一步支持 我们假设F-8-F-NH 2可能在 抗伤害感受 在大鼠中，发现F-8-F-NH 2也很高 集中在脑下垂体的后叶 抓紧搜救 对于这种肽在垂体中的作用，发现F-8-F-NH 2 低于脑垂体中的检测限 布拉特波罗大鼠尿崩症大鼠。 结果表明 Brattleboro大鼠可能是一个有用的模型， 垂体F-8-F-NH 2 采用免疫组化技术， 大鼠脑内强F-8-F-NH_2免疫反应神经元群 被揭露了 其中1例位于脑室周围 下丘脑区和另一个突出的细胞群被发现在 孤束核 这些细胞体是否投射到 脑垂体和脊髓目前正在研究中。 本研究拟探讨1） 使用Brattleboro大鼠的垂体F-8-F-NH 2，2）F-8-F-NH 2的释放， NH 2从脊髓中的释放以及吗啡对这种释放的影响 （3）F-8-F-NH_2免疫反应神经末梢的来源 脊髓和垂体的损伤。

The octapeptide. Phe-Leu-Phe-Gln-Arg-Phe-NH2 (F-8-F-NH2) was first detected by the antiserum raised against the molluscan cardioexcitatory peptide. Phe-Met-Arg-Phe-NH2, and subsequently isolated from bovine brain. F-8-F-NH2 injected intraventriculary can decrease the analgesic effect of Morphine. F-8-F-NH2 was found to be highly localized in substantia gelatinosa of dorsal spinal cords. In the study using in vitro and in vivo superfusion of spinal cords, we found that F-8-F-NH2 can be released by depolarizing concentration of KCI (in vitro) and also by substance P, the sensory transmitter (in vivo). This result further support our hypothesis that F-8-F-NH2 may have a modulatory role in antinociception. In rats, F-8-F-NH2 was found to be also highly concentrated in posterior lobes of pituitary glands. In searching for the role of this peptide in the pituitary, F-8-F-NH2 was found to be below the limit of detection in pituitary glands of Brattleboro rats, diabetes insipidus rats. The result indicates that Brattleboro rat may be an useful model to explore the function of pituitary F-8-F-NH2. Using Immunohistochemical technique, two strongly F-8-F-NH2 immunoreactive neuronal groups in rat brains were revealed. One of them was located in the periventricular hypothalamic area and another prominent cell group was found in the nucleus tratus solitarii. Whether these cell bodies project to pituitaries and spinal cord are under current investigation. The proposed course of this study is to investigate 1) function of pituitary F-8-F-NH2 using Brattleboro rats, 2) release of F-8-F- NH2 from the spinal cord and the effect of morphine on this release and 3) sources of F-8-F-NH2 immunoreactive nerve terminals in spinal cords and pituitaries using lesion techniques.