MET-ENKEPHALIN-ARG-PHE AND PHE-MET-ARG-PHE-NH2 IN THE BRAIN AND SPINAL CORD

此前，我们已经提出了几条证据，表明 哺乳动物中枢神经系统中Phe-Met-Arg-Phe-NH2样肽(S)可能 对阿片类抗伤害性感觉有调节作用。FMRF-NH2是一种 然而，文蛤来源的神经肽，存在FMRF-NH2样肽， 它们与四肽FMRF-NH2不同，现在哺乳动物中枢神经系统中 人所共知。为了进一步探讨内源蛋白在其中可能的作用 阿片类抗伤害作用中的FMRF-NH2样免疫反应，我们已经确定 对哺乳动物FMRF-NH2样多肽进行化学表征。两部小说 对FMRF-NH2抗血清有免疫反应的多肽已经被 从牛脑及其氨基酸序列中提纯到同源性 下定决心。此外，这两种多肽(八肽和八肽) 合成了十八肽)，并测定了其生物活性 老鼠。这两种多肽在脑室注射时都被发现 缩短大鼠甩尾潜伏期。其中一种多肽，八肽， 能显著减弱吗啡的镇痛作用。 该新药抗阿片作用的分子机制 哺乳动物的FMRF-NH2样肽目前正在研究中。

Previously, we have presented several lines of evidence suggesting that Phe-Met-Arg-Phe-NH2-like (FMRF-NH2-like) peptide(s) in mammalian CNS may have modulatory role in opiate antinociception. FMRF-NH2 was a neuropeptide of clam origin, however, presence of FMRF-NH2-like peptides, which are distinct from the tetrapeptide FMRF-NH2, in mammalian CNS is now well known. In order to further explore the possible role of endogenous FMRF-NH2-like immunoreactivity in opiate antinociception, we have decided to chemically characterize the mammalian FMRF-NH2 like peptides. Two novel peptides, which are immunoreactive to FMRF-NH2 antiserum, have been purified to homogeneity from bovine brain and their amino acids sequences determined. Furthermore, these two peptides (octapeptide and octadecapeptide) were synthesized and biological activities were tested in rats. Both peptides, when injected intraventricularly, were found to decrease tail flick latencies of rats. One of the peptides, octapeptide, was found to attenuate the analgesia induced by morphine significantly. The molecular mechanism underlying this antiopiate effect of the novel mammalian FMRF-NH2 like peptide is under current investigation.