MASS SPECTROMETER SYSTEM AND ACCESSORIES

质谱仪系统和配件

• 批准号: 3520733
• 负责人: ALAN S. NIES
• 金额: $ 18.6万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-07-06 至 1991-07-05
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3520733
• 关键词: biomedical equipment purchase; gas chromatography mass spectrometry

This proposal is for the purchase of a gas chromatography/mass spectrometry/data system that is capable of operating in both chemical ionization, negative ion mode (GC/NICI-MS) and electron impact ionization, positive ion mode. This instrument will be utilized by five investigators who are supported by the National Institutes of Health. Four of the projects to be supported are currently funded by the NIH and one represents a new direction for which funding is pending. The investigators and projects are as follows: (1) Alan S. Nies, M.D. (R01 HL 21308) "The role of Prostaglandins in the Local Control of the Circulation". This proposal is to investigate the role of prostacyclin in mediating the effects of antihypertensive drugs. Prostacyclin productions in humans will be assessed by the urinary excretion of the major enzymatic metabolite of prostacyclin, dinor-6-keto-PGF 1alpha as measured by GC/NICI-MS. The second part of this proposal is to investigate the renal release of platelet activating factor, which will be analyzed by GC/NICI- MS. (2) Duane C. Bloedow, Ph.D. (R01 GM 29543) "Hepatic Clearance of Drugs in Burn Patients". This proposal is to investigate the pharmacokinetics of model compounds, lidocaine and antipyrine, in burn patients. The analysis requires the simultaneous administration of [2H2] lidocaine orally, and an unlabelled form intravenously. The analyses of lidocaine and antipyrine and their metabolites will use mass spectrometry. (3) Keith L. Clay, Ph.D. (P01 HL 34303) "Ether Lipids, Eicosanoids, and Lung Cell Pathophysiology". This proposal requires GC/NICI-MS for sensitive assays of platelet activating factor and eicosanoids at very low concentrations. (4) Joseph Gal, Ph.D. (R01 HL/GM 34789) "Stereospecific Analysis of new Antiarrhythmic Agents". This proposal is to develop GC/MS methodology for the analysis of mexiletine enantiomers in serum in order to determine the stereochemical aspects of disposition when mexiletine (a racemic mixture) is administered. (5) John G. Gerber, M.D. (R01 DK 38504) "Modulators of Gastric Histamine Release in vivo". This project examines the factors controlling the release of gastric histamine in the dog and requires the use of GC/NICI-MS for the assay of histamine and methylhistamine in plasma in levels of 50-100pg/ml. The available instrumentation for analyses of large numbers of samples as generated by these five projects is limited. These projects cannot proceed at an optimal rate without the instrumentation requested. The University of Colorado has a commitment to mass spectrometry and the existing Mass Spectrometry Resource Center has offered its services for training personnel and for consultation on maintenance of the requested equipment. For this proposal, the University has committed funds for renovation of space and maintenance of the requested instrument in order to ensure its optimal utilization.

本建议书用于购买气相色谱/质谱仪 光谱/数据系统，能够在两种化学 离子化、负离子模式（GC/NICI-MS）和电子碰撞离子化， 正离子模式。 该仪器将由五名调查员使用 他们得到了美国国立卫生研究院的支持。 中的四 目前支持的项目由NIH资助， 这是一个新的方向，资金尚未到位。 的 （1）Alan S. Nies，医学博士（R01） HL 21308）“前列腺素在局部控制中的作用， 循环”。 本研究旨在探讨前列环素在 介导抗高血压药物的作用。 前列环素产量 将通过主要酶的尿排泄来评估人体内的 前列环素的代谢产物，去甲-6-酮-PGF 1 α，通过 GC/NICI-MS。本建议的第二部分是研究肾脏 血小板活化因子的释放，将通过GC/NICI进行分析- 女士 (2)杜安角Bloedow博士(R01 GM 29543）“肝脏清除率 烧伤病人的药物”。 这项建议是为了调查 烧伤模型化合物利多卡因和安替比林的药代动力学 患者 分析需要同时给予[2 H2] 口服利多卡因和静脉注射未标记形式。 的分析 利多卡因和安替比林及其代谢物将使用质谱法。 (3)基思湖Clay博士(P01 HL 34303）“醚类脂质、类花生酸和 肺细胞病理生理学”。 本提案要求GC/NICI-MS用于 血小板活化因子和类花生酸在极低 浓度的 (4)Joseph Gal博士(R01 HL/GM 34789）“立体特异性 分析新的抗肿瘤药物”。 本建议是发展GC/MS 血清中美西律对映体的分析方法 以确定当mexilobenzene（a） 外消旋混合物）。 (5)John G. Gerber，医学博士(R01 DK 38504） 胃组织胺释放的调节剂体内。 本项目研究 控制狗胃组胺释放的因素， 要求使用GC/NICI-MS测定组胺， 血浆中的甲基组胺水平为50- 100 pg/ml。 可用 用于分析大量样品的仪器， 这五个项目是有限的。 这些项目不能以 最佳速率而无需所需的仪器。 大学 科罗拉多致力于质谱分析和现有的质谱 光谱资源中心提供培训服务 人员和咨询所需设备的维护。 为此，联合国大学已承诺提供资金， 所需仪器的空间和维护，以确保其 最佳利用