GENETIC CONTROL OF SISTER CHROMATID EXCHANGE IN YEAST

酵母姐妹染色单体交换的遗传控制

• 批准号: 3898082
• 负责人: M A RESNICK
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3898082
• 关键词: DNA repair; Saccharomyces; fungal genetics; gene complementation; gene mutation; genetic manipulation; genetic recombination; genetic regulation; molecular cloning; molecular genetics; mutant; natural gene amplification; radiation genetics

The mechanism of sister chromatid recombination is poorly understood at the genetic and molecular level. Since it may be important in repair, mutagenesis and gene amplification, we have initiated studies to characterize the process and investigate the genetic control in the yeast Saccharomyces cerevisiae. Sister chromatid recombination is measured by recombination between two fragments of the HIS3 gene which share a 300 base pair region of homology. They are arranged in such a way that recombination will generate a complete gene. We have shown that several of the genes involved in DNA repair are required for the levels observed in wild type cells. Mutations in genes that enhance or depress homologous chromosome recombination proces. DNA damaging agents can considerably enhance the process although the cells are resistant to x-ray induced exchange. In order to determine more accurately the levels of spontaneous sister chromatid recombination, we have developed procedures for greatly reducing the background levels. This is allowing small increases to be determined more accurately.

姐妹染色单体重组的机制较差。 在基因和分子水平上被理解。因为它可能是 在修复、突变和基因放大方面很重要，我们有 启动研究以表征这一过程并调查 酿酒酵母的遗传控制。姐妹 染色单体的重组是通过两个染色体之间的重组来衡量的 HIS3基因的片段，这些片段共享300个碱基对的 同源。它们以这样一种方式排列，重组将 产生一个完整的基因。我们已经证明了有几个基因 参与DNA修复是在野外观察到的水平所必需的 键入单元格。增强或抑制同源基因的突变 染色体重组过程。DNA损伤剂可以 大大增强了过程，尽管细胞具有抵抗力 至x射线诱导的交换。为了更准确地确定 自发姐妹染色单体重组的水平，我们有 制定了大大降低背景水平的程序。 这使得小幅加薪得以更准确地确定。