NEUROBIOLOGIC STUDIES OF NEURONS AND GLIA IN CELL CULTURE

细胞培养中神经元和神经胶质细胞的神经生物学研究

• 批准号: 3898461
• 负责人: P G NELSON
• 金额: --
• 依托单位: EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3898461
• 关键词: adrenergic receptor; axon; calcium channel; calcium channel blockers; cell cell interaction; developmental neurobiology; electrophysiology; electrostimulus; embryo /fetus tissue /cell culture; ganglions; glia; glutamate receptor; intercellular connection; laboratory mouse; membrane channels; mixed tissue /cell culture; neural plasticity; neural transmission; neurons; neurophysiology; neurotransmitters; synapses

Dissociated cell cultures of fetal mouse spinal cord (SC) and dorsal root ganglion (DRG) neurons have been used in conjunction with a 3-compartment culture system to study the process of activity-dependent synapse augmentation and elimination. With this system, two independent populations of DRG neurons converge on and synapse with a common population of SC neurons. Chronic (3-4 days) electrical stimulation of one set of DRG axons results in this population establishing stronger synaptic connections with the target SC neurons relative to the convergent non-stimulated DRG axons. The NMDA receptor antagonist, APV, blocks this plasticity, but in solutions with raised calcium ion concentration (3 mM vs. 1.8 mM) plus AVP, stimulation again produces stronger synaptic connections from the stimulated axons as compared to those from the non-stimulated convergent axons. We infer a critical role for an increased intracellular calcium in the process of activity-dependent synapse competition.

胎鼠脊髓和背根的分离细胞培养 神经节（DRG）神经元已经与3-隔室结合使用， 研究活动依赖性突触过程的培养系统 增强和消除。 通过这个系统，两个独立的DRG神经元群体收敛于 并与共同的SC神经元群体形成突触。慢性（3-4天） 一组DRG轴突的电刺激导致该群体 与靶SC神经元建立更强的突触连接 相对于会聚的非刺激的DRG轴突。 NMDA受体拮抗剂，APV，阻断这种可塑性，但在 具有升高的钙离子浓度的溶液（3 mM vs. 1.8 mM）加上 AVP，刺激再次产生更强的突触连接， 刺激的轴突相比，那些从非刺激会聚 轴突我们推断细胞内钙离子的增加在 活动依赖性突触竞争的过程。