NEUROBIOLOGIC STUDIES OF NEURONS AND GLIA IN CELL CULTURE

细胞培养中神经元和神经胶质细胞的神经生物学研究

• 批准号: 3942010
• 负责人: P G NELSON
• 金额: --
• 依托单位: EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3942010
• 关键词: autoradiography; axon; calcium; calcium channel blockers; dorsal root; electrophysiology; embryo /fetus tissue /cell culture; ganglions; gel electrophoresis; glia; histochemistry /cytochemistry; ion transport; membrane permeability; membrane potentials; mixed tissue /cell culture; neural transmission; neurons; neuropharmacology; neurophysiology; neurotransmitters; radiotracer; spinal cord; synapses

A voltage-sensitive calcium channel agonist, BayK 8644, and its active isomer, R5417, increases calcium currents in spinal cord (SC) and dorsal root ganglion (DRG) neurons in cell culture under voltage clamp, particularly those currents which are activated between -40 and zero mV membrane potential. These agents do not increase excitatory transmitter output in this system, suggesting that the types of calcium channels sampled in the neuronal cell body may be distinct from those involved in transmitter release from neuronal terminals. Physiological studies of synapse formation between neurons in different compartments of a 3-compartment culture system have begun in conjunction with morphological observations described in Project Z01 HD 00708-03. Chronic stimulation of DRG axons making synaptic connections to SC neurons appear to increase the relative efficacy of these stimulated synapses.

电压敏感性钙通道激动剂BayK 8644及其 活性异构体R5417增加脊髓钙电流 (SC)和背根神经节（DRG）神经元在细胞培养下， 电压钳位，特别是那些被激活的电流 在-40和0 mV膜电位之间。 这些探员知道 不增加该系统中的兴奋性递质输出， 这表明，在心脏中取样的钙通道类型 神经元细胞体可能不同于参与 神经元末梢释放递质。 神经元间突触形成的生理学研究 三室培养系统的不同室具有 开始与形态学观察， 项目Z 01 HD 00708-03。 DRG轴突的慢性刺激 使突触连接到SC神经元似乎增加了 这些受刺激的突触的相对功效。