NEUROBIOLOGIC STUDIES OF NEURONS AND GLIA IN CELL CULTURE

细胞培养中神经元和神经胶质细胞的神经生物学研究

• 批准号: 3878029
• 负责人: P G NELSON
• 金额: --
• 依托单位: EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3878029
• 关键词: adrenergic receptor; afferent nerve; axon; calcium channel; calcium channel blockers; cell cell interaction; developmental neurobiology; electrophysiology; electrostimulus; embryo /fetus tissue /cell culture; ganglions; glia; glutamate receptor; intercellular connection; laboratory mouse; membrane channels; membrane potentials; mixed tissue /cell culture; neural plasticity; neural transmission; neurons; neuropharmacology; neurophysiology; neurotransmitters; synapses

A compartmental tissue culture system has been used to control neuronal activity, to measure the strength of synaptic connections between defined neuronal populations and test hypotheses as to the mechanisms involved in activity-dependent synaptic plasticity. Postsynaptic electrical activity, NMDA receptor activation and intracellular calcium levels interact in determining the long term changes in synaptic efficacy produced by chronic stimulation of synaptic inputs. Axonal outgrowth (growth cone activity) from DRG neurons is quickly blocked by electrical activation (phasic stimulation is more effective than tonic). With prolonged stimulation (hours), the growth cone can become accommodated and resume outgrowth. Electrical stimulation increases intracellular calcium in growing axons and their growth cones. The increase is transient and the calcium buffering capacity of axons appears to be regulated, in part, by electrical activity. BayK 8644 reliably augments high-voltage activated (HVA) calcium currents while omega-conotoxin produces a moderate decrease in these currents in cultured mouse spinal cord (SC) and DRG neurons. BayK 8644 augments synaptic interactions between these cell types in a minority of cases (no change in most), while omega-conotoxin is without detectable effect (omega-conotoxin eliminates synaptic transmission at the frog neuromuscular junction). Our results suggest a high degree of heterogeneity with regard to the calcium channels responsible for neurotransmitter release.

细胞间室组织培养系统已被用于控制神经元