NEUROBIOLOGIC STUDIES OF NEURONS AND GLIA IN CELL CULTURE

细胞培养中神经元和神经胶质细胞的神经生物学研究

• 批准号: 3965719
• 负责人: P G NELSON
• 金额: --
• 依托单位: EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3965719
• 关键词: Herpesviridae; acyclovir; autoradiography; axon; calcium; calcium channel blockers; dorsal root; electrophysiology; embryo /fetus tissue /cell culture; ganglions; gel electrophoresis; glia; histochemistry /cytochemistry; ion transport; membrane permeability; mixed tissue /cell culture; neural transmission; neurons; neuropharmacology; neurophysiology; neurotransmitters; radiotracer; spinal cord; synapses

It has become clear that several species of voltage sensitive calcium channels (VSCC) exist in different tissues and even within the same cell. The different types of these channels can be distinguished on the basis of their voltage and pharmacological sensitivities. We have addressed the question of which types of calcium channels may be involved in neurotransmitter release at central synapses. Nitrendipine, a blocker of VSCC in muscle does not affect neuronal VSCC and similarly has no detectable effect on transmitter release. BayK 8644 is a VSCC agonist increasing one type of VSCC, the L channel, in dorsal root ganglion (DRG) neurons. This agent does not increase transmitter output from either DRG or spinal cord (SC) synaptic terminals. This suggests that the L channel is not involved in transmitter release. DRG and SC neurons and muscle cells have been grown in a three-compartment chamber tissue culture system. Selective growth of axons into chambers containing target tissue as well as synapse formation by axons growing from one chamber to another have been demonstrated.

已经很明显，有几种电压敏感钙 通道存在于不同的组织中，甚至存在于同一细胞内。 这些频道的不同类型可以基于以下各项来区分 它们的电压和药理敏感性。我们已经解决了 可能涉及哪些类型的钙通道的问题 中枢突触的神经递质释放。尼群地平，一种阻滞剂 肌肉中的VSCC不影响神经元VSCC，同样也没有 对发射机释放的可检测影响。Bayk 8644是一种VSCC激动剂 在背根神经节增加一种类型的VSCC--L通道 神经元。此代理不会增加这两个DRG的发射机输出 或脊髓(SC)突触终末。这表明L渠道 不参与发射器的释放。 背根神经节和SC神经元和肌肉细胞生长在一个三室的房间里 室内组织培养系统。轴突选择性生长入室 包含靶组织以及由生长的轴突形成的突触 从一个房间到另一个房间已经被证明了。