PHENOTYPING OF HUMAN CYTOCHROME P-450

人细胞色素 P-450 的表型分析

• 批准号: 3939641
• 负责人: F K FRIEDMAN
• 金额: --
• 依托单位: DIVISION OF CANCER EPIDEMIOLOGY AND GENETICS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3939641
• 关键词: antiserum; carcinogen testing; chemical carcinogenesis; cytochrome P450; drug interactions; ethanol; human tissue; liver metabolism; microsomes; monoclonal antibody; neoplasm /cancer diagnosis; neoplastic cell; nitrosamines; radioimmunoassay

The individual forms of cytochrome P-450 display unique substrate specificity and reactivity profiles toward a variety of drugs and carcinogens. Differences in cytochrome P-450 phenotype may relate to individual differences in sensitivity to certain drugs and susceptibility to carcinogenesis. Monoclonal antibodies (MAbs) to cytochromes P-450 have been used as specific probes for the cytochromes P-450 in human liver. Western blot analysis with antisera to rat ethanol-induced P-450, a form with high nitrosamine metabolizing activity, detected a P- 450 in human liver homogenates. Individual variation in the level of this P-450 was observed. MAb 1-98-1 to this rat P-450 also detected a related human P-450 in liver microsomes by Western blot analysis. A radioimmunoassay for P-450 in human liver microsomes was developed using this MAb. Further refinement will provide a rapid, efficient method for screening large numbers of samples from human tissues.

细胞色素P-450的单个形式显示出独特的 底物特异性和反应性特征， 药物和致癌物质。 细胞色素P-450 表型可能与个体差异的敏感性， 某些药物和致癌易感性。 单克隆 针对细胞色素P-450的抗体（MAb）已经被用作 人肝细胞色素P-450的特异性探针。 用大鼠乙醇诱导的P-450的抗血清进行Western印迹分析， 一种具有高亚硝胺代谢活性的形式，检测到P- 450人肝匀浆中。 水平的个体差异 P-450被发现 MAb 1-98-1对该大鼠P-450的作用也 Western blot检测到肝微粒体中的相关人P-450 印迹分析 人肝P-450的放射免疫测定 使用该单克隆抗体开发微粒体。 进一步细化 将提供一种快速有效的方法来筛选大量 从人体组织中提取的样本