PHENOTYPING OF HUMAN CYTOCHROME P-450

人细胞色素 P-450 的表型分析

• 批准号: 3963440
• 负责人: F K FRIEDMAN
• 金额: --
• 依托单位: DIVISION OF CANCER EPIDEMIOLOGY AND GENETICS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3963440
• 关键词: alpha benzopyrone; chemical carcinogenesis; cytochrome P450; detoxification; drug interactions; enzyme substrate; ethanol; gene expression; human tissue; immunochemistry; liver; lymphocyte; methylcholanthrene; microsomes; monoclonal antibody; monocyte; neoplasm /cancer diagnosis; neoplastic cell; phenobarbital; radioimmunoassay; surface antigens; unspecific monooxygenase

The individual forms of cytochrome P-450 display unique substrate specificity and reactivity profiles toward a variety of drugs and carcinogens. Differences in cytochrome P-450 phenotype may relate to individual differences in sensitivity to certain drugs and susceptibility to carcinogenesis. Monoclonal antibodies (MAbs) to cytochromes P-450 have been used as specific probes for epitope-specific cytochromes P-450 in human liver samples obtained from different individuals. Western blot analysis with antisera or a MAb to rat ethanol-induced P-450 detected a P-450 in human liver homogenates and microsomes. The level of the immunodetectable P-450 varied among livers from different individuals. A human liver P-450 was immunopurified using the MAb and was structurally characterized. In addition, we are developing a radioimmunoassay for human cytochrome P-450 as a rapid, efficient method for screening large numbers of samples from human tissues.

细胞色素P-450的个体形式显示出独特的底物