PHENOTYPING OF HUMAN CYTOCHROME P-450

不同形式的细胞色素P-450显示独特的底物 多种药物和药物的特异性和反应性 致癌物质。细胞色素P-450表型的差异可能与 对某些药物的敏感度的个体差异 对化学致癌的敏感性。单克隆抗体 (单抗)对各种形式的大鼠肝细胞色素P-450已被 用作人肝细胞色素P-450的特异性探针。 用单抗对乙醇诱导的大鼠P-450进行Western印迹分析 具有较高的亚硝胺代谢活性，检测到一种相关的P- 450在人肝微粒子中。水平上的个体差异 其中P-450被观察到。用放射免疫分析法(RIA)测定该P- 450是用MAB 1-98-1研制的。使用这种分析方法，我们能够 检测和定量检测人单抗特异性细胞色素P-450 肝脏微粒体。对人的P-450蛋白进行免疫纯化和分析 通过肽图谱和氨基酸序列测定。

The individual forms of cytochrome P-450 display unique substrate specificity and reactivity profiles toward a variety of drugs and carcinogens. Differences in cytochrome P-450 phenotype may relate to individual differences in sensitivity to certain drugs and susceptibility to chemical carcinogenesis. Monoclonal antibodies (MAbs) to various forms of rat liver cytochrome P-450 have been used as specific probes for the cytochrome P-450s in human liver. Western blot analysis with MAb to rat ethanol-induced P-450, a form with high nitrosamine metabolizing activity, detected a related P- 450 in human liver microsomes. Individual variation in the level of this P-450 was observed. A radioimmunoassay (RIA) to this P- 450 was developed with MAb 1-98-1. Using this assay we were able to detect and quantitate the MAb-specific cytochrome P-450 in human liver microsomes. This human P-450 was immunopurified and analyzed by peptide maps and amino acid sequencing.