IMMUNOPURIFICATION AND CHARACTERIZATION OF CYTOCHROME P-450

细胞色素 P-450 的免疫纯化和表征

基本信息

项目摘要

The cytochromes P-450 metabolize a wide array of compounds, including xenobiotics such as drugs and carcinogens, and endogenous compounds such as steroids. The focus of this project is the characterization of structure-function relationships and regulation of the multiple forms of this enzyme. Monoclonal antibodies (MAbs) to rat P-450s are a tool in these studies. A 3- methylcholanthrene (MC)-inducible P-450 was immunopurified from the livers and lungs of rats. On the basis of amino acid sequence analysis, peptide mapping, and molecular weight, the liver and lung P-450s were indistinguishable. Using a MAb to ethanol-inducible rat liver P-450, a P-450 has been purified from both rat and human liver. These differed in primary structure as evidenced by different amino terminal sequences and peptide maps. Developmental regulation of P-450 was examined by studying P-450-dependent testosterone metabolism in 3- and 24- month old rats. Ring hydroxylation patterns, as well as content of liver constitutive P-450s, varied with age. While P-450 activities generally declined with age, the 7 alpha-hydroxylase and corresponding P-450 form responsible for this activity increased with age.
细胞色素P-450代谢多种化合物,

项目成果

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F K FRIEDMAN其他文献

F K FRIEDMAN的其他文献

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{{ truncateString('F K FRIEDMAN', 18)}}的其他基金

STRUCTURE FUNCTION OF CYTOCHROME P450
细胞色素 P450 的结构功能
  • 批准号:
    2463623
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
IMMUNOPURIFICATION AND CHARACTERIZATION OF CYTOCHROME P-450
细胞色素 P-450 的免疫纯化和表征
  • 批准号:
    4692374
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
PHENOTYPING OF HUMAN CYTOCHROME P-450
人细胞色素 P-450 的表型分析
  • 批准号:
    3939641
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
STRUCTURE-FUNCTION OF CYTOCHROME P-450
细胞色素 P-450 的结构-功能
  • 批准号:
    3838351
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
PHENOTYPING OF HUMAN CYTOCHROME P-450
人细胞色素 P-450 的表型分析
  • 批准号:
    3916767
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
STRUCTURE FUNCTION OF CYTOCHROME P450
细胞色素 P450 的结构功能
  • 批准号:
    6100796
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
IMMUNOPURIFICATION AND CHARACTERIZATION OF CYTOCHROME P-450
细胞色素 P-450 的免疫纯化和表征
  • 批准号:
    3963471
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
PHENOTYPING OF HUMAN CYTOCHROME P-450
人细胞色素 P-450 的表型分析
  • 批准号:
    3963440
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
STRUCTURE-FUNCTION OF CYTOCHROME P450
细胞色素 P450 的结构-功能
  • 批准号:
    5201473
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
STRUCTURE AND REGULATION OF CYTOCHROME P-450
细胞色素 P-450 的结构和调控
  • 批准号:
    3916787
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
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