IMMUNOPURIFICATION AND CHARACTERIZATION OF CYTOCHROME P-450
细胞色素 P-450 的免疫纯化和表征
基本信息
- 批准号:3939659
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:
- 资助国家:美国
- 起止时间:至
- 项目状态:未结题
- 来源:
- 关键词:N hydroxylation aminoacid analyzer animal age group chemical carcinogenesis chemical structure function cytochrome P450 drug interactions drug metabolism enzyme linked immunosorbent assay ethanol genetic mapping hormone metabolism human tissue isozymes liver metabolism lung methylcholanthrene molecular weight monoclonal antibody steroid hormone metabolism testosterone toxin metabolism
项目摘要
The cytochromes P-450 metabolize a wide array of compounds,
including xenobiotics such as drugs and carcinogens, and
endogenous compounds such as steroids. The focus of this project
is the characterization of structure-function relationships and
regulation of the multiple forms of this enzyme. Monoclonal
antibodies (MAbs) to rat P-450s are a tool in these studies. A 3-
methylcholanthrene (MC)-inducible P-450 was immunopurified
from the livers and lungs of rats. On the basis of amino acid
sequence analysis, peptide mapping, and molecular weight, the
liver and lung P-450s were indistinguishable. Using a MAb to
ethanol-inducible rat liver P-450, a P-450 has been purified from
both rat and human liver. These differed in primary structure as
evidenced by different amino terminal sequences and peptide
maps. Developmental regulation of P-450 was examined by
studying P-450-dependent testosterone metabolism in 3- and 24-
month old rats. Ring hydroxylation patterns, as well as content of
liver constitutive P-450s, varied with age. While P-450 activities
generally declined with age, the 7 alpha-hydroxylase and
corresponding P-450 form responsible for this activity increased
with age.
细胞色素P-450代谢多种化合物,
项目成果
期刊论文数量(0)
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会议论文数量(0)
专利数量(0)
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{{ truncateString('F K FRIEDMAN', 18)}}的其他基金
IMMUNOPURIFICATION AND CHARACTERIZATION OF CYTOCHROME P-450
细胞色素 P-450 的免疫纯化和表征
- 批准号:
4692374 - 财政年份:
- 资助金额:
-- - 项目类别:
IMMUNOPURIFICATION AND CHARACTERIZATION OF CYTOCHROME P-450
细胞色素 P-450 的免疫纯化和表征
- 批准号:
3963471 - 财政年份:
- 资助金额:
-- - 项目类别: