QUANTITATIVE STUDIES ON CONCURRENT FACTORS IN NEOPLASTIC TRANSFORMATION

继续研究小鼠的转化机制 胚胎细胞系，BALB/3 T3克隆A31-1-1。 改造是 用几种金属制成。 三价和 五价砷（As）显示，三价砷是 比五价砷更活泼。 然而，当与 计算的As摄取和细胞负荷、毒性和转化 频率被认为是相同的两种形式的作为。 的 代谢研究结果表明，三价砷是 负责毒性和转化效应，独立 原子价态的变化。 BALB/3 T3细胞被金属和有机致癌物转化， 或自发地，当接种在裸鼠中时， 致瘤性但不转移，即使静脉注射 局 这一发现与缺乏类型一致 在这些转化细胞中观察到IV胶原溶解活性 线 通过用以下物质进一步治疗诱导转移活性 ras癌基因或致癌物正在调查中。

Studies were continued on mechanisms of transformation in the mouse embryo cell line, BALB/3T3 clone A31-1-1. Transformation was obtained with several metals. Studies with trivalent and pentavalent arsenic (As) showed that trivalent As was four times more active than pentavalent As. However, when correlated with the calculated As uptake and cell burden, toxicity and transformation frequency were found to be the same for both forms of As. The results of metabolic studies indicate that trivalent As is the form responsible for the toxic and transforming effects, independently of the valence state of the As exposure. BALB/3T3 cells transformed by metals and by organic carcinogens, or spontaneously, when inoculated in nude mice were found to be tumorigenic but not metastasizing, even by intravenous administration. This finding is consistent with the lack of type IV collagenolytic activity observed in these transformed cell lines. Induction of metastatic activity by further treatment with the ras oncogene or with carcinogens is under investigation.