INTERACTION OF GTP-BINDING MEMBRANE PROTEINS WITH CELLULAR COMPONENTS

GTP 结合膜蛋白与细胞成分的相互作用

• 批准号: 3916565
• 负责人: R A KAHN
• 金额: --
• 依托单位: DIVISION OF CANCER TREATMENT
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3916565
• 关键词: adenosine diphosphate; adenylate cyclase; binding proteins; cell components; cell growth regulation; glycosylation; guanine nucleotide binding protein; guanine nucleotides; guanosine triphosphate; hydrolysis; membrane proteins; nucleotide metabolism; oncogenes; oncoproteins

Activation of the ras oncogene has been implicated as the causative agent in as many as 30% of all human tumors. Yet in spite of extensive work on the ras gene, almost nothing is known about the biochemical function of the ras proteins. A recently characterized protein, the ADP-ribosylation factor or ARF, is a component of the adenylate cyclase system and shares several features with ras, p21 including size, location, and the ability to bind GTP. Characterization of the binding and hydrolysis of guanine nucleotides by ras and ARF will be undertaken. A systematic search will then be conducted to identify factor(s) which increase either the exchange or hydrolysis of guanine nucleotides by these regulatory proteins. The assays developed in these studies will also be used to help define the function of specific genes, identified in yeast as suppressors or activators of ARF disruption. These studies should help locate cellular targets for these regulatory proteins and may identify the physiological role of these proteins in cellular metabolism or proliferation.

ras癌基因的激活被认为是导致 多达30%的人类肿瘤。 然而，尽管 尽管对ras基因进行了大量的研究，但人们对ras基因几乎一无所知。 ras蛋白的生化功能。 最近， ADP核糖基化因子（ADP-ribosylation factor，ARF）是一种蛋白质， 腺苷酸环化酶系统，并与ras，p21 包括大小、位置和结合GTP的能力。 鸟嘌呤的结合和水解的表征 将进行ras和ARF的核苷酸测定。 了系统性检索 然后将进行识别因素，增加 鸟嘌呤核苷酸的交换或水解 调节蛋白 在这些研究中开发的测定将 也可以用来帮助确定特定基因的功能， 在酵母中鉴定为ARF破坏的抑制剂或激活剂。 这些研究应该有助于定位这些细胞的目标， 调节蛋白，并可能确定的生理作用， 这些蛋白质参与细胞代谢或增殖。