GROWTH AND INTERACTION OF ORAL MICROORGANISMS

口腔微生物的生长和相互作用

• 批准号: 3917117
• 负责人: S A ROBRISH
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3917117
• 关键词: Fusobacterium; aminoacid metabolism; carbohydrate transport; fermentation; galactose; gel electrophoresis; glucose; glutamates; glycogen; growth media; histidine; lysine; microorganism culture; microorganism growth; microorganism metabolism; oral bacteria; sodium; trehalose

The carbohydrate storage product made from glucose or galactose by F. nucleatum proved to be a polyglucose or glycogen. The use of the glycogen, made from either glucose or galactose, was spared when energy was available to the washed cells by fermenting amino acids. Glutamate, lysine and histidine, the amino acids previously shown to stimulate sugar transport, were the same ones which anaerobically suppressed glycogen use. Aerobically the amino acids had no effect on the loss of label from glycogen previously synthesized from glucose or galactose. Analysis of intermediates during glycogen use revealed that glucose was used by the Embden- Meyerhoff pathway by an organism which lacks a phosphoenolpyruvate phosphotransferase sugar transport system. Analysis of fermentation products obtained from cells using labeled preformed glycogen revealed that butyric acid was a product of the fermentation of sugars as well as amino acids. Washed cells from a culture of F. nucleatum, grown on a sugar free complex medium, were able to transport glucose or galactose without a lag if the anaerobic cell suspension was incubated with glutamate before the addition of the sugar. Sodium is important in the glutamate mediated stimulation of glucose transport and/or, glycogen synthesis by F. nucleatum. The ammonium salt of glutamic acid would not effectively stimulate glucose or galactose use unless sodium chloride was included in the incubation mixture. In addition, ammonium glutamate would not be used anaerobically by a cell suspension of F. nucleatum without sodium chloride.

碳水化合物由葡萄糖或半乳糖通过 F.核质被证明是多糖或糖原。 使用 由葡萄糖或半乳糖组成的糖原被保留下来 当通过发酵氨基为洗涤后的细胞提供能量时 acids. 谷氨酸，赖氨酸和组氨酸，这些氨基酸 先前显示刺激糖运输的， 其厌氧抑制糖原的使用。 有氧氨基 酸对先前糖原标记的损失没有影响 由葡萄糖或半乳糖合成。 中间体分析 在糖原使用过程中，显示葡萄糖被恩布登使用， 缺乏磷酸烯醇丙酮酸的微生物的Meyerhoff途径 磷酸转移酶糖转运系统。 分析 使用标记的预成型的发酵产物从细胞获得的发酵产物 糖原显示，丁酸是一个产品的 糖和氨基酸的发酵。 清洗细胞， F. nucleatum，生长在无糖复合培养基上， 能够运输葡萄糖或半乳糖而没有滞后， 厌氧细胞悬浮液与谷氨酸一起孵育， 添加糖。 钠在谷氨酸介导的刺激中是重要的， 葡萄糖转运和/或F.具核的 的 谷氨酸铵盐不能有效地刺激 葡萄糖或半乳糖使用，除非氯化钠包括在 孵育混合物。 此外，谷氨酸铵不会 由F.的细胞悬浮液厌氧使用。无核细胞 氯化钠