GROWTH AND INTERACTION OF ORAL MICROORGANISM

口腔微生物的生长和相互作用

• 批准号: 4692657
• 负责人: S A ROBRISH
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4692657
• 关键词: Fusobacterium; Streptococcus sanguis; aminoacid metabolism; butyrates; cell cell interaction; glucose metabolism; growth media; microorganism culture; oral bacteria

Pure and mixed cultures of the coaggregating pair; Fusobacterium nucleatum and Streptococcus sanguis (CC5A) have been grown successfully in both the batch and continuous modes. Pure culture studies revealed that F. nucleatum used glutamic acid and glucose concurently during exponential growth in batch culture while less than half the glucose, and all of the glutamic acid were used in continuous culture. Glutamic acid seemed to be used in preference to glucose by F. nucleatum. Lysine was used after glucose and glutamic acid during batch growth of F. nucleatum. A stable mixed culture, including extensive coaggregation of these organisms was obtained when CC5A was inoculated into the continuous culture medium before F. nucleatum. In contrast, using the same conditions, the growth of CC5A was suppressed if F. nucleatum was inoculated first. When growth together in continuous culture, F. nucleatum was reduced to very low levels while CC5A became dominant as the dilution rate was raised. F. nucleatum remained in the culture and its increase was reflected by an increased butyric acid production after the dilution rate of the culture was reduced. Butyric acid production proved directly related to the growth rate of F. nucleatum. In pure culture, two rates of cell growth and butyric acid formation were seen, while in a batch grown mixed culture, only a single rate of butyric acid production was observed. The interactions of the two organisms during butyrate production are unknown and under investigation.

共聚集对的纯培养物和混合培养物;具核梭杆菌 和血链球菌（CC 5A）已成功地生长在这两个 批量和连续模式。 纯培养研究表明，F. nucleatum在指数生长期同时利用谷氨酸和葡萄糖 生长在分批培养，而不到一半的葡萄糖，和所有的 谷氨酸用于连续培养。 谷氨酸似乎是 F.优先使用葡萄糖。具核的 赖氨酸在 葡萄糖和谷氨酸在F.具核的 稳定的混合培养，包括这些细胞的广泛共聚集 将CC 5A接种到连续培养中，获得了一株 在F.具核的 相比之下，使用相同的条件， F.先接种核质。 当 在连续培养中共同生长，F.核质减少到很低 随着稀释率的增加，CC 5A逐渐占优势。 F. 有核质仍然存在于培养物中，其增加反映在 培养物稀释后丁酸产量增加 减少了。 丁酸产量与F的生长速度直接相关。 具核的 在纯培养中，两种细胞生长速率和丁酸 形成，而在一批生长的混合培养物中，只有一个 观察丁酸产生速率。 两者的互动 丁酸盐生产过程中的微生物是未知的，正在调查中。