GROWTH AND INTERACTION OF ORAL MICROORGANISMS

口腔微生物的生长和相互作用

• 批准号: 3854198
• 负责人: S A ROBRISH
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3854198
• 关键词: 6 phosphofructokinase; Fusobacterium nucleatum; acetates; aminoacid metabolism; anaerobic bacteria; carbohydrate biosynthesis; fructose; galactose; gel electrophoresis; glucose; glucose metabolism; glucose transport; glutamates; glycolysis; growth media; lactates; microorganism culture; microorganism growth; microorganism metabolism; oral bacteria; phosphorylation

Amino acid dependent glucose accumulation, previously demonstrated only in Fusobacterium nucleatum (10953), was examined in representative species of fusobacteria. An absolute amino acid energy requirement for glucose accumulation by anaerobically maintained washed cells was demonstrated for most of the species tested except F. necrophorum and F. mortiferum. F. mortiferum accumulated glucose without an amino acid addition, however, almost twice the glucose was accumulated when serine was included; while F. necrophorum accumulated a much smaller fraction of the glucose without an amino acid. F. mortiferum grew well with a wide variety of sugars added to a complex peptone medium in contrast to the other fusobacteria tested. F. mortiferum accumulated glucose, fructose, galactose, mannose, sucrose and maltose and accumulated the sugar best on which it had been grown. Glucose use was constitutive with the sugar accumulated actively be serine grown cells while only maltose grown F. mortiferum would accumulate maltose. The differences in accumulation of the sugars probably reflects the induction of individual sugar permeases because the organism has a common fermentation path and separate kinases have been demonstrated. F. mortiferum accumulates a glycogen like storage material as demonstrated in electron micrographs of thin sections of the organism. The intensity of the granular storage product observed was dependent on the sugar used for growth: galactose, the most and fructose the least. Constitutive kinase activities were demonstrated for glucose, fructose, and mannose in cell extracts from serine or sugar grown F. mortiferum. Purification of the kinase activity revealed a separable glucokinase from a fructokinase which would also phosphorylate mannose. The molecular weight, amino acid sequence, and metal ion requirements of a highly purified F. mortiferum fructokinase revealed that the protein was very similar to a fructokinase isolated by other members of this laboratory from Lactococcus lactis.

氨基酸依赖性葡萄糖积累，以前仅在 具核梭杆菌（10953），检查了代表性的物种， 梭菌 葡萄糖的绝对氨基酸能量需求 通过厌氧维持的洗涤细胞的积累被证明是 除F. necrophorum和F.死亡 F. 然而，在没有添加氨基酸的情况下， 当包括丝氨酸时，几乎两倍的葡萄糖被积累;而 F. necrophorum积累了一小部分葡萄糖， 氨基酸。 F. mortiferum生长良好，有各种糖 添加到复合蛋白胨培养基中，与其他梭菌相反 测试. F. mortiferum积累葡萄糖，果糖，半乳糖，甘露糖， 蔗糖和麦芽糖，并积累了最好的糖， 长大了 葡萄糖的利用是由糖的积极积累构成的 F.死亡将 积累麦芽糖。 糖积累的差异 可能反映了个别糖渗透酶的诱导，因为 生物体具有共同的发酵途径， 演示。 F.死亡菌积累类似糖原的储存物质 如有机体薄切片的电子显微照片所示。 观察到的颗粒状储存产物的强度取决于 用于生长的糖：半乳糖最多，果糖最少。 对葡萄糖、果糖， 和甘露糖在丝氨酸或糖生长的F.死亡 激酶活性的纯化揭示了一种可分离的葡萄糖激酶， 一种果糖激酶，也可以磷酸化甘露糖。 分子 重量，氨基酸序列和金属离子的要求， 纯化的F. mortiferum果糖激酶显示，该蛋白非常 类似于本实验室其他成员分离的果糖激酶 来自乳酸乳球菌