MOLECULAR BIOLOGY OF GLYCOPROTEIN HORMONES

人促甲状腺激素(TSH)β亚基基因已被分离并 已排序。它不同于以前分离的啮齿动物TSH-β 启动子组织中的基因，这可能具有重要的 对人类启动子功能的影响。这种基因已经被 在哺乳动物细胞中表达，并通过与过量的共转染 α亚基c DNA，生物活性促甲状腺激素已被 制作。目前，我们正在研究 了解基因启动子区域对促甲状腺激素的调控 人类的生产。同样，通过定点突变， 我们正在探索编码的结构-功能关系 基因的不同区域。 通过检测促甲状腺激素释放激素研究下丘脑对促甲状腺激素的调节 不同年龄和不同内分泌的啮齿动物体内的信使RNA 状态。我们观察到下丘脑因素很重要。 在脑下垂体对甲状腺功能减退的反应。 全身性甲状腺激素抵抗综合征 限制性片段长度与人c-erbaβ基因的连锁 多态分析。这意味着贝塔基因编码了 一种对人体甲状腺激素起重要作用的受体。

The human thyrotropin (TSH) beta subunit gene has been isolated and sequenced. It differs from previously isolated rodent TSH-beta genes in promotor organization, which may have important implications for promotor function in man. The gene has been expressed in mammalian cells, and by co-transfection with excess alpha subunit cDNA, biologically active thyrotropin has been produced. Currently we are studying regulatory elements in the promotor region of the gene to understand the control of TSH production in man. Similarly, through site directed mutagenesis we are exploring structure-function relationships of the coding regions of the gene. Hypothalamic regulation of TSH is being studied by examining TRH messenger RNA in rodents of various ages and different endocrine status. We have observed that hypothalamic factors are important in the pituitary response to hypothyroidism. The syndrome of generalized thyroid hormone resistance has been linked to the human c-erbA beta gene by restriction fragment length polymorphism analysis. This implies that the beta gene codes for a receptor important for thyroid hormone action in man.