BIOSYNTHESIS AND GLYCOSYLATION OF THYROTROPIN

糖蛋白构成了最常见的一类病毒和 原生动物包膜蛋白、哺乳动物血清蛋白、血浆 膜受体、转运体和离子通道。尽管它 已经提出，这些蛋白质可能具有异质性 碳水化合物的结构、特殊的化学性质和 这种异质性的重要性尚不清楚。使用新的 发展的糖肽凝集素分析方法以及 高效液相色谱(HPL C)分析 酶释放糖，我们已经阐明了至少9种不同的 分泌型促甲状腺激素的碳水化合物变体。此外，对于第一次 在我们已经阐明的任何糖蛋白系统中的时间 这种糖基化的发育和内分泌调节，以及 目前正在研究这种受调控的功能相关性 异质性。这些研究可能会揭示一种新的后 可以解释多个方面的翻译控制机制 甲状腺生理学和病理生理学。

Glycoproteins constitute the most common class of viral and protozoal envelope proteins, mammalian serum proteins, plasma membrane receptors, transporters and ion channels. Although it has been suggested that such proteins may have heterogeneity of carbohydrate structures, the specific chemical nature and significance of such heterogeneity is not known. Using newly developed methods of lectin analysis of glycopeptides as well as high performance liquid chromatography (HPLC) analysis of enzyme-released sugars, we have elucidated at least 9 different carbohydrate variants of secreted TSH. Moreover, for the first time in any glycoprotein system we have elucidated major developmental and endocrine regulation of such glycosylation, and are currently studying the functional correlates of such regulated heterogeneity. These studies may reveal a novel post- translational control mechanism that may explain various aspects of thyroid physiology and pathophysiology.