CLONING OF THE CDNA FOR AVIAN INTESTINAL EPITHELIAL BRUSH BORDER CALDESMON

钙调素是一种细丝钙调素结合蛋白， 似乎在肌动蛋白-肌球蛋白的调节中起作用 平滑肌和非肌肉细胞的相互作用。 存在 钙调素的至少2种亚型，一种发现的高分子量形式 主要存在于平滑肌中， 主要是在非肌肉细胞中。 我们正在尝试克隆和测序 鸡肠刷状缘中发现钙调素。 一旦这个 我们想用细菌表达载体 研究表达的蛋白质， 变体。

Caldesmon is a thin filament calmodulin-binding protein which appears to play a role in the regulation of actin-myosin interaction in smooth muscle and nonmuscle cells. There are at least 2 isoforms of caldesmon, a high molecular weight form found primarily in smooth muscle and a lower molecular weight form found mainly in nonmuscle cells. We are attempting to clone and sequence the caldesmon found in chicken intestinal brush border. Once this is accomplished we would like to use bacterial expression vectors and study the expressed protein as well as genetically engineered variants.