CHOLESTEROL INDUCED EFFECTS ON BRUSH BORDER MEMBRANE

胆固醇对刷状缘膜的影响

• 批准号: 6121596
• 负责人: MOSHE LEVI
• 金额: $ 0.14万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6121596
• 关键词: biomedical resource; human tissue; lipids

Our studies were conducted to determine (1) the effects of cholesterol on renal proximal tubular apical brush border membrane fluidity and ion transport activity, both in renal tubular cells grown in culture, and in isolated brush border membranes; (2) the role of alterations in membrane cholesterol and phospholipid composition and membrane fluidity in adaptation of renal tubular cell phosphate transporter to acute and chronic perturbations in extracellular phosphate concentration; and (3) the role of alterations in membrane lipid composition and fluidity in the response of the renal phosphate transport to extracellular hormones. Consulting: 36 hours with Weiming Yu to help set-up new 2-photon computer and Fluorescence microscope, on site. Also installed computer hardware and software for microscope

我们的研究是为了确定(1) 胆固醇对肾近端小管尖刷状缘膜的影响 肾小管上皮细胞的流动性和离子转运活性 在培养和隔离的刷子边缘膜中；(2) 膜胆固醇和磷脂组成的变化及 肾小管细胞磷酸盐适应过程中的膜流动性 细胞外急、慢性扰动的转运蛋白 磷酸盐浓度；以及(3)膜变化的作用 肾磷酸盐反应中的脂质成分和流动性 运输到细胞外荷尔蒙。咨询：36小时 于伟明将帮助建立新的双光子计算机和荧光 显微镜，现场。还安装了计算机硬件和软件 用于显微镜