Mechanisms of Peptide Transport In Human Intestinal Brush Border Membrane Vesicles

人肠刷状缘膜囊泡肽转运机制

• 批准号: 9018027
• 负责人: Diane Wilson
• 金额: $ 1.2万
• 依托单位: University of Delaware
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-02-01 至 1993-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9018027&HistoricalAwards=false
• 关键词: Mechanisms; Peptide; Transport; Human; Intestinal

The goal of this research is to study the mechanism of peptide transport in human small intestine using brush border membrane vesicles. Presently, there is no information on the specificity and multiplicity of the peptide transport systems located on human small intestine. The specific aims are: 1. To determine total uptake of the peptide substrate. 2. To investigate the kinetics of peptide transport. 3. To classify the structural characteristics of the peptide transporter. The investigator proposes to conduct a series of experiments starting with the isolation of the human intestinal brush border membrane vesicles obtained from organ donations. Uptake of labeled substrates will be determined by the rapid filtration method and hydrolysis of the peptides will be monitored using high performance liquid chromatography. The simple diffusion component of the peptides will be determined by treating each peptide as a competitive inhibitor of its own mediated transport. Experiments will be conducted to determine the kinetic characteristics of peptide uptake. Various dipeptides and tripeptides will be used as inhibitors in order to determine the specificity of transport.

本研究的目的是研究 人小肠内肽转运的刷法研究 边缘膜小泡。 目前，没有 的具体性和多样性的资料， 肽转运系统位于人类小 肠。 具体目标是： 1.测定肽底物的总摄取。 2.研究肽转运的动力学。 3.对结构特征进行分类， 肽转运蛋白 调查人员建议进行一系列 实验从隔离人类开始 肠刷状缘膜囊泡， 器官捐赠 标记底物的摄取将 通过快速过滤法和水解法测定 的肽将使用高性能 液相色谱法 的简单扩散分量 通过处理每种肽 作为其自身介导的运输的竞争性抑制剂。 将进行实验以确定动力学 肽摄取的特征。 各种二肽和 三肽将用作抑制剂， 确定运输的特异性。