OBSERVATIONS ON CEREBRAL ISCHEMIA AT INJURY THRESHOLD LEVELS

损伤阈值水平脑缺血的观察

• 批准号: 3922629
• 负责人: M SEIDA
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3922629
• 关键词: brain cell; brain edema; brain injury; cats; cell death; cerebral artery; cerebral ischemia /hypoxia; cerebrovascular occlusions; disease /disorder model; model design /development; neurons; nonsurgical revascularization

Studies on mechanisms, associated with survival of the neurons subjected to ischemia, have important clinical implications, since elucidation of the nature of such mechanisms may ameliorate or prevent brain tissue damage following cerebral ischemic insults. In experimental research, the degree of ischemic injury which allows recovery of affected neurons is called "penumbra", i.e. resembling a twilight zone around solar eclipse. In our studies, we produced conditions of penumbra by subjecting cats to 20 minute occlusion of the middle cerebral artery (MCA) and studying various parameters of ischemic injury up to 2 weeks after the insult. Our studies demonstrated that although the animals showed significant and widespread changes up to 3 days after recirculation, there was either complete or partial recovery with regard to edema and neuronal damage, when animals were examined 2 weeks after MCA occlusion. Our investigations resulted thus in establishing an experimental model where pathomechanisms associated with reversible ischemic damage could be analyzed and, potentially, manipulated.

与神经元存活相关的机制研究 遭受缺血，具有重要的临床意义，因为 阐明此类机制的性质可能会改善或 预防脑缺血损伤后的脑组织损伤。 在实验研究中，缺血性损伤的程度 允许受影响的神经元恢复称为“半影”，即 类似于日食周围的暮光区。 在我们的研究中， 我们通过让猫经历 20 分钟来创造半影条件 大脑中动脉（MCA）闭塞并研究各种 损伤后 2 周内的缺血性损伤参数。 我们的 研究表明，尽管动物表现出显着的 再循环后 3 天发生广泛变化， 水肿完全或部分恢复 MCA 后 2 周检查动物时神经元损伤 闭塞。 我们的调查结果是建立了一个 实验模型，其中病理机制与可逆相关 缺血性损伤可以被分析，并且有可能被操纵。