JEOL 1200EX TRANSMISSION ELECTRON MICROSCOPE

JEOL 1200EX 透射电子显微镜

• 批准号: 3519862
• 负责人: PETER STERLING
• 金额: $ 19万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-02-29 至 1989-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3519862
• 关键词: biomedical equipment purchase; cell biology; electron microscopy; histochemistry /cytochemistry; morphology

Funds are requested to purchase a JEOL 1200 EX electron microscope to be housed in the EM facility of the Department of Anatomy. This instrument would be devoted primarily to studies of serial sections in analyzing: 1) local neuronal circuits in mammalian brain 2) regeneration of identified neural circuits in vertebrate spinal cord 3) macromolecular organization of the myosin filament. The technical features of the new instrument critical to these purposes are: 1) high accelerating voltage (120KV) 2) excellent performance at low magnification (approximately 1500X) as well as high magnification (10,000-100,000X) 3) advanced aids to critical focusing 4) microprocessor controlled realignment for efficient switching between different magnifications (for efficient time sharing by different users) 5) video system to permit scanning at low beam intensities (to minimize beam damage and contamination during search for corresponding regions of serial sections and during alignment of serial sections for photography) 6) capability of rotating and tilting the sections in the column (for alignment during photography and for producing stereo pairs of critical structures) 7) automatic montage photography. The instrument would also serve other members of the department in studies of motility and cell differentiation The proposed instrument would replace a 15 year old instrument in the Department of Anatomy which is technologically obsolete and increasingly unstable and unreliable.

请拨资金购买一台JEOL 1200 EX型电子显微镜 将被安置在解剖学系的EM设施中。 这 一份文书将主要用于研究连续各节 在分析中：1）哺乳动物大脑中的局部神经元回路2） 脊椎动物脊髓神经回路的再生 索3）肌球蛋白丝的大分子组织。 的 新文书的技术特点对这些目的至关重要 主要有：1）加速电压高（120KV）2）性能优良 在低放大率（约1500倍）以及高 3）先进的辅助设备，以关键的 聚焦4）微处理器控制的重新对准， 在不同的放大率之间切换（为了有效的时间 由不同用户共享）5）视频系统，以允许在 低光束强度（以使光束损坏和污染最小化 在搜索连续部分的对应区域期间， 在连续切片的对齐过程中进行摄影）6）能力 旋转和倾斜柱中的部分（用于对齐 在摄影过程中，并产生立体对的关键 7）自动蒙太奇摄影。 该文书将 也服务于部门的其他成员在运动的研究 所提议的仪器将取代一个 15岁的仪器在解剖部门， 技术过时，越来越不稳定和不可靠。