GENETIC CONTROL OF SISTER CHROMATID EXCHANGE IN YEAST

姐妹染色单体重组的机制尚不清楚

The mechanism of sister chromatid recombination is poorly understood at the genetic and molecular level. Since it may be important in repair, mutagenesis and gene amplification, we have initiated studies to characterize the process and investigate the genetic control in the yeast Saccharomyces cerevisiae. Sister chromatid recombination is measured by recombination between two fragments of the HIS3 gene which share a 300 base pair region of homology. They are arranged in such a way that recombination will generate a complete gene. We have shown that several of the genes involved in DNA repair are required for the levels observed in wild type cells. Mutations in genes that enhance or depress homologous chromosome recombination have similar function son the sister chromatid recombination process. DNA damaging agents can considerably enhance the process although the cells are resistant to x-ray induced exchange. Mutants have been isolated which can either lead to enhanced levels (esr) or depressed (dsr) levels of exchange. Three mutants (esr1, esr2, esr3) have been characterized extensively. Each of these mutants behaves like a single Mendelian locus. esr1 and esr2 have elevated levels of mitotic gene conversion. Experiments are underway to clone the esr1, esr2, and esr3 genes by complementation.