IDENTIFICATION AND ISOLATION OF C-FMS PROTOONCOGEN FROM F344/N RAT LEUKEMIA

F344/N 大鼠白血病 C-FMS 原癌原的鉴定和分离

• 批准号: 3941497
• 负责人: J E FRENCH
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3941497
• 关键词: carcinogenesis; cell bank /registry; cell differentiation; gene expression; growth factor; macrophage; neoplasm /cancer genetics; neoplastic cell; oncogenes; phagocytes

The proto-oncogene, c-fms, has been described in both mature cat macrophages and undifferentiated mouse and human tumors, and normal human bone marrow cells. The c-fms proto-oncogene is expressed during human monocyte differentiation and the c-fms product has been related to the receptor for mononuclear phagocyte growth factor (CSF-1). The purpose of this investigation is: (1) to screen for the presence of the c-fms proto-oncogene in the in vivo serially transplanted F344/N mononuclear leukemic cell lines and, (2) to initiate studies on the isolation and expression of the gene product. This will provide insight on the origin of the tumor and aid in providing diagnostic criteria for the tumor. In addition, this tumor line may provide a valuable source of genetic material for investigations into the molecular biology of the c-fms protooncogene and its potential effect on MNCL expression. Preliminary investigation and tentative conclusions indicate the possible presence of c-fms oncogene isolated from in vivo transplanted MNCL tumor lines.

原癌基因 c-fms 已在成熟猫中得到描述 巨噬细胞和未分化的小鼠和人类肿瘤，以及 正常人骨髓细胞。 c-fms原癌基因是 在人类单核细胞分化和 c-fms 过程中表达 产品与单核受体有关 吞噬细胞生长因子（CSF-1）。 本次调查的目的是：（1）筛查是否存在 c-fms原癌基因在体内连续移植的研究 F344/N 单核白血病细胞系，以及 (2) 启动研究 基因产物的分离和表达。 这将 提供有关肿瘤起源的见解并帮助提供 肿瘤的诊断标准。 此外，该肿瘤系可能 为研究提供宝贵的遗传物质来源 c-fms 原癌基因的分子生物学及其 对 MNCL 表达的潜在影响。 初步调查 初步结论表明 c-fms 可能存在 从体内移植的 MNCL 肿瘤系中分离出癌基因。