ULTRAVIOLET RADIATION INDUCED SKIN CANCER

工作总结：UV-B辐射可能会抑制诱导和 迟发型超敏反应(DTH)的诱发。我们已经开发出一种 激光多普勒血流成像定量分析方法 UV-AB对小鼠迟发型超敏反应的诱导效应 以前的结核菌素致敏的个体，以调查 紫外线剂量与抑制诱导的剂量反应关系 或对紫外线诱导抑制的抵抗力。19名健康志愿者 每天在上背部勾画的区域进行为期四天的照射 有UV-AB辐射。调整UV-AB剂量水平，以提供60%或 120 mJ/cm2 UV-B辐射。第四天，一个被照射，一个被照射 在每个紫外线剂量上随机选择未辐照的测试地点 个人和皮内注射结核菌素。测试反应 并在96h进行临床和DPI分析。这个 结果表明，UV-AB辐射能抑制诱发期 有剂量依赖性和个体化 紫外线辐射对免疫抑制作用的差异。处于低谷 剂量UV-B，观察到敏感和抗性表型在 1：1的比例。然而，加倍的UV-B增加了对某些 个体，在其他抵抗的个体中诱导抑制，或者 在其他人身上诱导了一种抗性或耐受性 较低剂量的紫外线。我们假设紫外线的阈值会导致 抑制迟发型超敏反应和诱导可能阻断的T抑制细胞 启发性差异很大。因此，对UVR的描述 和UVR剂量-反应关系，以诱导易感性或 必须确定抗性，才能充分表现出这些 特征。

Summary of Work: UV-B radiation may suppresses the induction and elicitation of delayed-type hypersensitivity (DTH). We have developed a quantitative method using laser Doppler perfusion imaging analysis (DPI) of UV-AB induced suppression of DTH using the elicitation response in previously tuberculin sensitized individuals in order to investigate the dose response relationship between UV dose and induction of suppression or resistance to UV induced suppression. Nineteen healthy volunteers were irradiated daily for four days on delineated areas on the upper back with UV-AB radiation. UV-AB dose levels were adjusted to deliver 60 or 120 mJ/cm2 UV-B radiation. On the fourth day, one irradiated and one unirradiated test site for each UV-dose were chosen at random on each individual and injected intradermally with tuberculin. Test reactions were analyzed and quantified at 96 h clinically and with DPI. The results indicate that UV-AB radiation can suppress the elicitation phase of tuberculin DTH and that there are dose dependent and individual differences in the immune suppressive effect of the UV-radiation. At low dose UV-B, both the susceptible and resistant phenotypes were observed at an ratio of 1:1. However, doubling UV-B increased the suppression in some individuals, induced suppression in others that were resistant, or induced a resistance or tolerance in others that were susceptible at the lower dose of UV. We hypothesize that the thresholds for UV induced suppression of DTH and the induction of T suppressor cells that may block elicitation vary considerably. Thus, characterization of both the UVR and the UVR dose response relationship for induction of susceptibility or resistance must be determined in order to adequately phenotype for these traits.