TRANSMISSION ELECTRON MICROSCOPE

我们正在申请这笔资金，以购买我们急需的 这是一台带有电子显微镜的仪器 无失真成像和高速加速能力 高对比度高倍率传输电压 电子显微镜。利用免疫电子显微镜和 透射电子显微镜辅助心脏形态测量， 肺和分离的细胞我们希望获得新的和有用的 肺内免疫生物学反应的研究进展 对氧毒性的反应，C5a诱导的炎症和 高血压心脏病。通过利用免疫电子 显微镜我们计划在分子水平上研究这些组件 肺内C5转换酶与膜攻击的关系 络合物C5-9。此外，我们还将研究结构 细胞溶解T细胞的特性及其与T细胞的相互作用 靶肿瘤细胞，因为它与其细胞溶解颗粒有关 内容。 这些项目都是由美国国立卫生研究院资助的。实现这些目标 研究项目将得到极大的加强 已请求TEM。我们也希望获得新的信息 关于C5-C5转换酶相互作用的化学计量学 和C5-C9络合物形成。 我们所有研究的最终目标是获得数据，为 有用的信息，最终可能导致开发 预防和治疗模式。

We are applying for the funds to purchase a much needed instrument, a Transmission Electron Microscope with the capabilities for distortion free imaging and high accelerating voltage for high contrast high magnification transmission electron microscopy. Utilizing immunoelectron microscopy and transmission electron microscopy aided morphometry of hearts, lungs and isolated cells we hope to gain new and useful information in the immunobiological reactions in the lung in response to oxygen toxicity, C5a induced inflammation and in hypertensive heart diseases. By utilizing immunoelectron microscopy we plan to study at molecular levels the assemblies of C5 convertase in the lung and the membrane attack complexes C5-9. Additionally, we will study the structural features of the cytolytic T cells and their interactions with the target tumor cells, as it pertains to their cytolytic granule content. These projects are NIH funded grants. Fulfillment of these research projects will be greatly enhanced by availability of the TEM requested. We also hope to obtain new information regarding the stoichiometry of C5-C5 convertase interactions and of the C5-C9 complex formation. Ultimate goal of all our research is to obtain data to provide useful information that can ultimately lead to development of preventive and therapeutic modes.