ERROR-PRONE REPAIR IN BACTERIOPHAGE T4

这个项目的中心目标是分析容易出错的修复 在噬菌体T4中，与其他机制的相关探索， 诱变和进入一个新的机制，以避免紫外线诱导的 杀人 T4中的大多数化学诱变和所有辐射诱变都是通过 并且依赖于基因uvsW、uvsX和 uvsY，DNA复制所需的大部分或全部基因， 还有其他一些基因。 我们最近发现 X和Y基因的温度敏感等位基因， 差异地影响诱变和失活。 我们正在克隆 这些突变基因，以获得足够量的蛋白质， 为了确定哪些动力学参数与诱变相关，以及哪些动力学参数与诱变相关， 与生存。 uvsW的温度敏感等位基因也被发现。 获得，并将其特征在于生存和突变后， 与此同时，一个克隆的uvsW基因正在被修剪， 为了进一步研究其结构和功能， 这个基因。 我们计划探索哪些其他基因可能需要 T4中的易错修复，特别注意基因46，47，49，58 和59，并继续进行定位突变的作图实验， HM，其促进紫外诱变。 实验将在 以确定易错修复和基因重组是否 这两个过程都是由WXY途径的基因控制的。 最后，我们最近发现了一种新的紫外线后生存模式 辐射将准备出版;该系统涉及 编码ssDNA结合蛋白和解旋酶蛋白的基因。

The central objective of this project is the analysis of error-prone repair in bacteriophage T4, with related explorations into other mechanisms of mutagensis and into a novel mechanism for avoiding ultraviolet-induced killing. Most chemical and all radiation mutagenesis in T4 occurs via error-prone repair and depends on the functions of the genes uvsW, uvsX and uvsY, most or all of the genes required for DNA replication, and perhaps several other genes as well. We have recently characterized temperature-sensitive alleles of the X and Y genes, alleles that differentially affect mutagenesis and inactivation. We are now cloning these mutant genes in order to obtain their proteins in amounts sufficient to determine which kinetic parameters correlate with mutagenesis and which with survival. Temperature-sensitive alleles of uvsW have also been obtained and will be characterized as to survival and mutation after UV irradiation; at the same time, a clone of the uvsW gene is being trimmed down to minimum size for further studies of the structure and function of this gene. We plan to explore which other genes may be required for error-prone repair in T4, with particular attention to genes 46, 47, 49, 58 and 59, and to continue with mapping experiments to localize a mutation, hm, which promotes ultraviolet mutagenesis. Experiments will be conducted to determine whether error-prone repair and genetic recombination are correlated, both processes being controlled by genes of the WXY pathway. Finally, our work on a recently discovered new mode of survival after UV irradiation will be prepared for publication; this system involves the genes encoding the ssDNA-binding and helicase proteins.