CHARACTERIZATION OF A CORONAVIRUS FROM RABBITS

兔子冠状病毒的特征

• 批准号: 3965224
• 负责人: J D SMALL
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3965224
• 关键词: Coronaviridae; antibody neutralization test; antibody specificity; antiviral antibody; blood tests; creatine kinase; electrocardiography; enzyme linked immunosorbent assay; myocardium; myocardium disorder; nucleic acid sequence; respiratory virus; tissue /cell culture; virus classification

Our objective is to study the pathogenesis of rabbit coronavirus (RbCV), the rabbit's physiologic response to this virus, and the relatedness of RbCV to other members of the Coronaviridae. Serum neutralization studies against RbCV with antiserum to canine coronavirus (CCV), feline infectious peritonitis (FIP), and transmissible gastroenteritis (TGE) viruses in the intact rabbit were completed. Results were: CCV 2/3, FIP 2/3, TGE 1/3, RbCV 3/3 survived. RbCV alone was lethal. All rabbits receiving RbCV in combination with antiserum to one of the coronaviruses, including RbCV showed clinical signs of disease. As expected RbCV antiserum muted clinical signs the most. RbCV reacted with antisera to CCV, FIP, or TGE produced a typical clinical picture of disease. In some survivors the rectal temperature remained above 40 degrees C for 10-12 days and in others the temperature pattern closely followed that of rabbits receiving RbCV + RbCV antiserum. Gross lesions in those which died were similar to rabbits dying with RbCV alone. Gross lesions were not observed in survivors killed following recovery. Based on previous work showing a 2 way cross with coronavirus 229E (Human), partial cross protection from vaccination with CCV and FIP, and this new data, RbCV is most probably in Group II of the Coronaviridae. Nucleotide homology studies will be required to prove this supposition. Assessment of myocardial damage measuring creatine kinase isozymes are in progress. Further attempts will be made to adapt RbCV to tissue culture. The significance of this work lies in the ability to study a viral disease with a cardiotropism in an animal of sufficient but manageable size to allow sequential clinical and physiological observations. The damage to the rabbit heart by RbCV has a corollary in the human heart with the Coxsackie viruses, Mycoplasma pneumoniae, influenza virus, Herpes zoster, and possibly other infectious agents.

本研究的目的是研究兔冠状病毒（RbCV）的致病机制， 兔子对这种病毒的生理反应，以及 RbCV与冠状病毒科的其他成员。 血清中和研究 用抗犬冠状病毒（CCV）、猫传染性 腹膜炎（FIP）和传染性胃肠炎（TGE）病毒 完整家兔完成。 结果为：CCV 2/3，FIP 2/3，TGE 1/3， RbCV 3/3存活。 单用RbCV是致命的。 所有接受RbCV的家兔， 与一种冠状病毒（包括RbCV）的抗血清联合使用 显示出疾病的临床症状。 正如预期，RbCV抗血清减弱 临床症状最多。 RbCV与CCV、FIP或TGE抗血清反应 产生了典型的疾病临床图像。 在一些幸存者中， 直肠温度保持在40摄氏度以上10-12天， 体温模式与接受RbCV +的家兔密切相关 RbCV抗血清。 死亡动物的大体病变与家兔相似 死于RbCV 在被杀的幸存者中未观察到肉眼病变 恢复后。 基于先前的工作，显示了与 冠状病毒229 E（人），接种疫苗的部分交叉保护， CCV和FIP，以及这一新数据，RbCV最有可能在第II组中。 冠状病毒科 核苷酸同源性研究将需要证明这一点 假设 肌酸激酶测定对心肌损伤的评价 同工酶正在进行中。 将进一步尝试使RBCV适应 组织培养 这项工作的意义在于学习能力 一种病毒性疾病，在足够但 可管理的尺寸，以允许连续的临床和生理 意见。 RbCV对兔心脏的损害具有以下推论： 人类心脏感染了科萨基病毒，肺炎支原体， 流感病毒、带状疱疹和可能的其他传染性病原体。