CHARACTERIZATION OF A CORONAVIRUS FROM RABBITS

兔子冠状病毒的特征

• 批准号: 4693196
• 负责人: J D SMALL
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4693196
• 关键词: Coronaviridae; antibody neutralization test; antibody specificity; antiviral antibody; blood tests; creatine kinase; electrocardiography; enzyme linked immunosorbent assay; myocardium; myocardium disorder; nucleic acid sequence; respiratory virus; tissue /cell culture; virus classification

The objective of this project is to study the pathogenesis of rabbit coronavirus (RbCV), the rabbit's physiologic response to this virus, and the relatedness of RbCV to other members of the Coronaviridae. RbCV antiserum was shown to cross react strongly with transmissible gastroenteritis virus (TGE), canine coronavirus (CCV), and feline infectious peritonitis (FIP) in the RIA, slightly to TGE and CCV, but not FIP in a plaque serum neutralization and not at all in a gel diffusion test. Two of 3 rabbits vaccinated with CCV and l of 3 vaccinated with FIP survived challenge with RbCV. None vaccinated with TGE or calf diarrhea coronavirus (CDCV) survived challenge. Single rabbits given RbCV reacted with FIP antisserum or TGE antiserum did not survive. Based on previous work showing a 2 way cross with coronavirus 229E and this new data, RbCV is most probably in Group II of the Coronaviridae [229E (Human), TGE (swine), FIP (cat), CCV (dog)]. Nucleotide homology studies will be required to prove this supposition. Such studies are hampered by the present inability to grow this virus in tissue culture. Two of 3 rabbits given RbCV reacted with CCV antiserum for 2 hours survived. The apparent protective effect of CCV vaccination will be explored further. Additional studies to understand the relatedness of RbCV to the other coronaviruses will be done. Assessment of myocardial damage using ECGs and creatine kinase isozymes are in progress. In cooperation with a team of virologists, further attempts will be made to adapt RbCV to tissue culture. The significance of this work lies in the ability to study a viral disease with a cardiotropism in an animal of sufficient but manageable size to allow sequential clinical and physiological observations. The damage to the rabbit heart by RbCV has a corollary in the human heart with the Coxsackie viruses, Mycoplasma pneumoniae, influenza virus, Herpes zoster, and possibly over infectious agents.

本项目的目的是研究兔的发病机制。 冠状病毒(RbCV)，兔对该病毒的生理反应，以及 Rbcv与冠状病毒科其他成员的亲缘关系。RbCV 抗血清与传染性疾病有强烈的交叉反应。 胃肠炎病毒、犬冠状病毒和猫 RIA中的感染性腹膜炎(FIP)，对TGE和CCV轻微，但不 FIP在一个斑块中和血清，而在凝胶中完全不扩散 测试。3只兔中2只接种CCV疫苗，3只L接种FIP3只 在RbCV的挑战中幸存下来。没有接种过TGE或小牛腹泻疫苗 冠状病毒(CDCV)在攻击中存活。单只给予RbCV的兔出现反应 FIP抗血清或TGE抗血清均不能存活。基于以前的 研究显示了与冠状病毒229E的双向交叉，这一新数据，RbCV是 最可能属于冠状病毒科II组[229E(人)，TGE(猪)， FIP(猫)、CCV(狗)]。核苷酸同源性研究将被要求 证明这一假设。这样的研究由于目前的能力不足而受到阻碍。 在组织培养中培养这种病毒。注射RbCV的3只兔中有2只出现反应 接种CCV抗血清2小时后存活。 将探索接种CCV疫苗的明显保护作用 再远一点。更多研究以了解RbCV与 其他冠状病毒也会被做出来。应用超声心动图评价心肌损伤 ECGs和肌酸激酶同工酶正在进行中。与一家 病毒学家团队，将进一步尝试使RbCV适应组织 文化。 这项工作的意义在于能够研究一种病毒疾病 在一只体型足够但可管理的动物身上具有心脏亲和性 允许连续的临床和生理观察。造成的损害 RbCV制作的兔心在人类心脏中有一个推论 柯萨奇病毒，肺炎支原体，流感病毒，带状疱疹， 可能还有感染性物质。