CHARACTERIZATION OF A CORONAVIRUS FROM RABBITS

兔子冠状病毒的特征

• 批准号: 3941502
• 负责人: J D SMALL
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3941502
• 关键词: Coronaviridae; antibody neutralization test; antibody specificity; antiviral antibody; blood tests; creatine kinase; density gradient ultracentrifugation; electrocardiography; electron microscopy; isozymes; myocardium; myocardium disorder; respiratory virus; tissue /cell culture; virus classification

The objective of this project is to study the pathogenesis of rabbit coronavirus (RbCV), the rabbit's physiologic response to this virus, and the relatedness of RbCV to other members of the Coronaviridae. Serum neutralization studies against RbCV were extended using antiserum to human coronavirus (229-E). Two of 3 rabbits given RbCV treated with 229-E antiserum survived. All 3 exhibited clinical signs of disease but the 2 surviving rabbits had an accelerated recovery based on diminished eyes lesions and the body temperature returning to normal on post inoculation day 5 or 6. RbCV was purified on a sucrose density gradient and the bouyant density determined to be between 1.195 and 1.210. The bouyant density of 229-E determined at the same time was between 1.195 and 1.200. The RbCV recovered from the density gradient was infectious for rabbits and coronavirus like particles were seen by electron microscopy. Homology of RbCV and 229-E to transmissible gastroenteritis virus (TGEV) nucleic acid was demonstrated. This new data provides further proof for RbCV being a coronavirus of Group II related to 229-E. Purified RbCV is being used to make antibody in guinea pigs and rats for further studies of tissue tropisms. Assessment of myocardial damage by measurement of creatine kinase isozymes will be done. Attempts to adapt RbCV to tissue culture continue. Production of monoclonal antibodies and cloning of the RbCV genome are planned. The significance of this work lies in the ability to study a viral disease with a cardiotropism in an animal of sufficient but manageable size to allow sequential clinical and physiological observations. The damage to the rabbit heart by RbCV has a corollary in the human heart with the Coxsackie viruses. Mycoplasma pneumoniae, influenza virus, Herpes zoster, and possibly other infectious agents. Further, it has been estimated that approximately 10-30% of human "colds" are caused by coronaviruses.

本课题的目的是研究家兔肺结核的发病机制 冠状病毒（RbCV），兔子对这种病毒的生理反应， 以及RbCV与其他成员的相关性。 冠状病毒科 针对RbCV的血清中和研究如下： 使用抗人冠状病毒（229-E）的抗血清延伸。 三个中的两个 给予用229-E抗血清处理的RbCV的兔存活。 所有3 表现出疾病的临床症状，但2只存活的兔子 加速恢复的基础上减少眼睛病变和 接种后第5天体温恢复正常，或 6. 在蔗糖密度梯度上纯化RbCV， 浮力密度确定在1.195和1.210之间。 的 同时测定的229-E浮力密度为 在1.195和1.200之间。 从密度恢复的RbCV 梯度对家兔和冠状病毒样颗粒具有感染性 通过电子显微镜观察。 RbCV和229-E的同源性 对猪传染性胃肠炎病毒（TGEV）核酸的敏感性 演示。 这一新数据为RbCV提供了进一步的证据 是与229-E相关的II组冠状病毒。 纯化RbCV 用于在豚鼠和大鼠中制备抗体， 组织趋向性研究。 心肌损伤的评估 将进行肌酸激酶同工酶的测量。 尝试 使RbCV适应组织培养。 生产 单克隆抗体和RbCV基因组的克隆， 计划好了 这项工作的意义在于学习能力 一种病毒性疾病，在足够但 可管理的尺寸，以允许连续的临床和生理 意见。 RbCV对家兔心脏的损伤具有明显的 人类心脏中的科萨基病毒的必然结果。 肺炎支原体、流感病毒、带状疱疹和 可能还有其他传染源 此外，据估计， 大约10-30%的人类“感冒”是由 冠状病毒