CHARACTERIZATION OF AN ACID FAST BACTERIUM ISOLATED FROM ANIMAL DRINKING WATER

从动物饮用水中分离的抗酸细菌的表征

• 批准号: 4693199
• 负责人: J D SMALL
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4693199
• 关键词: Mycobacterium; drug administration routes; granuloma; liver disorder; lung disorder; microorganism classification; water microbiology; water pollution; water quality; water sampling /testing; water supply

The objective of this project is to characterize and study the pathogenic potential of an acid fast (AF) bacterium repeatedly isolated from an ultrapure water source furnishing drinking water to some of the Institute's animals. The organism has been identified as Mycobacterium chelonae. Inoculation of euthymic and athymic mice by IV, and euthymic mice by IP, IN, and gavage routes and in the drinking water produced lesions in the liver and, by the IN route, lesions in the lungs. No deaths were observed, even when mice were given cortisone acetate. Mycobacteria were recovered from livers of euthymic mice inoculated 7 days earlier but not thereafter. Granulomas were observed in the liver from post inoculation day (PID) 2 until day 35 when the experiment was terminated. The lesion was greatest in size and reaction in mice killed on PID 10 and 14. By day 35 lesions were well defined resolving granulomas; but, AF bacteria were rare. Acid fast bacteria were observed in livers of athymic mice on PID 50; however, no bacteria were recovered by culture and lesions were minimal or absent. The number of bacteria required to produce lesions will be examined as will ways to increase the likelihood of recovering the organism from the liver. Spontaneous liver lesions will be searched for AF organisms. Isolator maintained mice and rats will be compared to room maintained animals. Other water borne organisms will be examined for their ability to induce comprable liver lesions. This work is significant because of the many similar focal lesions of undetermined origin observed in livers of animals on chronic bioassays conducted under the NIP. Identifying waterborne bacteria and a Mycobacterium sp. In particular as the cause would allow for corrections in management practices.

该项目的目的是表征和研究致病性 抗酸（AF）细菌的潜力，反复分离自 超纯水水源供应饮用水的一些研究所的 动物 该微生物已被鉴定为龟分枝杆菌。 通过IV接种正常胸腺和无胸腺小鼠，通过IP接种正常胸腺小鼠， IN，灌胃途径和饮用水中产生的病变， 肝脏和通过IN途径的肺部病变。 未观察到死亡， 即使给老鼠注射醋酸可的松。 回收了分枝杆菌 来自7天前接种的正常胸腺小鼠的肝脏，但此后没有。 从接种后第2天（PID）开始，在肝脏中观察到肉芽肿 直到第35天实验结束。 病变最大 在PID 10和14处死的小鼠中的大小和反应。 第35天病变 明确定义的解决肉芽肿，但AF细菌是罕见的。 酸 在PID 50时在无胸腺小鼠的肝脏中观察到快速细菌;然而， 通过培养没有回收到细菌，并且损伤最小或不存在。 将检查产生病变所需的细菌数量， 增加从肝脏中回收有机体的可能性的方法。 将在自发性肝脏病变中搜索AF微生物。 隔离器 将饲养的小鼠和大鼠与室内饲养的动物进行比较。 将检查其他水生生物诱导 肝脏损害。 这项工作是有意义的，因为许多类似的局灶性病变， 慢性生物测定中在动物肝脏中观察到来源不明 在NIP下进行。 鉴定水生细菌和 特别是分枝杆菌，因为原因将允许纠正 在管理实践中。