A RESOURCE FOR BIOMEDICAL MASS SPECTROMETRY

华盛顿大学质谱研究资源计划： 1.为当地，区域， 国家和国际生物医学研究人员 2.提供专家技术援助、分析服务， 高精度GCMS选择离子监测解释建议 底物含量和稳定同位素富集的定量（SIM-GCMS） 在生理样本中。 3.提供全自动同位素比质谱仪（IRMS）， 用于测量稳定同位素的快速、大样品体积操作 内源性底物的过期和排泄终产物富集 新陈代谢. 4.建立了同位素比值监测气相色谱-质谱联用仪（IRM-GCMS）， 稳定同位素定量分析，样品量小， 比IRMS更少的样品制备，并且能够测量 比SIM-GCMS更大的示踪剂稀释度。 5.建立高分辨质谱-快速原子电离 用于分析高分子量或热不稳定物质的设备 分子。 6.开发一种高效液相色谱-热喷雾质谱仪， 常规方法进行肽测序并建立其他HPLC-MS 分析。 7.使新开发的设施可供合格的 地方、区域、国家和国际各级的调查人员。

Research at Washington University Mass Spectrometry Resource plans: 1. to provide wide range low resolution GCMS service to local, regional, national, and international biomedical investigators 2. to provide expert technical assistance, analytical services, and interpretive advice on high precision GCMS selected ion monitoring quantitation (SIM-GCMS) of substrate content and stable isotope enrichment in physiologic samples. 3. To provide a fully automated isotope ratio mass spectrometer (IRMS) for rapid, high sample volume operation for measurement of stable isotope enrichment in expired and excreted end products of endogenous substrate metabolism. 4. To develop an isotope ratio monitoring GCMS (IRM-GCMS) for high precision quantitative stable isotope analysis with smaller sample size and less sample preparation than with IRMS and with the ability to measure greater tracer dilution than possible with SIM-GCMS. 5. To establish a high resolution mass spectrometry-fast atom ionization facility for the analysis of high molecular weight or thermally labile molecules. 6. To develop a facility for HPLC-thermospray mass spectrometry to augment conventional methods for peptide sequencing and establish other HPLC-MS analyses. 7. To make the newly developed facilities available for use by qualified investigators at the local, regional, national, and international levels.