Differentiation of human fetal liver stem cells and pancreas derived progenitor cells to pancreatic beta-cells

人胎儿肝脏干细胞和胰腺来源的祖细胞分化为胰腺β细胞

• 批准号: G0901969/1
• 负责人: Robert Drummond
• 金额: $ 28.4万
• 依托单位: University of Edinburgh
• 依托单位国家: 英国
• 项目类别: Fellowship
• 财政年份: 2010
• 资助国家: 英国
• 起止时间: 2010 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=G0901969%2F1
• 关键词: Differentiation; human; fetal; liver; stem

In recent years there has been significant advances in the treatment of diabetes by the transplantation of replacement beta-cells (the type of cell destroyed in the course of the disease). At present however, the ability to perform cell transplantation is limited by the availability of suitable donor cells in significant numbers. This project aims to isolate a population of cells from the developing liver and pancreas that can be expanded in culture and may eventually be used to provide replacement cells in order to treat this condition.During fetal development, the pancreas (containing beta-cells) and the liver share a common developmental origin, suggesting that they may share some common stem/progenitor cells. Our project aims to isolate a potential type of progenitor cell based on the presence of a cell surface marker protein (Thy-1+). We then aim to compare cells from both fetal liver and pancreas for their capacity to change into beta-cells under specific culture conditions and to test and compare these cells grown in the laboratory to those found normally in the body.

近年来，通过移植替代β细胞（在疾病过程中被破坏的细胞类型）治疗糖尿病取得了重大进展。然而，目前进行细胞移植的能力受到大量合适供体细胞的可用性的限制。该项目旨在从发育中的肝脏和胰腺中分离出一群细胞，这些细胞可以在培养中扩增，最终可能用于提供替代细胞，以治疗这种疾病。在胎儿发育过程中，胰腺（含有β细胞）和肝脏具有共同的发育起源，这表明它们可能具有一些共同的干细胞/祖细胞。我们的项目旨在基于细胞表面标记蛋白（Thy-1+）的存在分离一种潜在类型的祖细胞。然后，我们的目标是比较胎儿肝脏和胰腺细胞在特定培养条件下转化为β细胞的能力，并将实验室培养的这些细胞与正常体内发现的细胞进行测试和比较。